ArticleInternational journal of molecular sciences2023
An Inferred Ancestral CotA Laccase with Improved Expression and Kinetic Efficiency.
Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Structural and functional characterisation of a reconstructed ancestral strigolactone receptor.The Plant journal : for cell and molecular biology · 2026Article
- Inferring an ancestral alginate lyase for improved stability and high-level expression using fed-batch fermentation.Frontiers in microbiology · 2026Article
- Molecular approaches for enhancing fermented bamboo-derived feed additives: A sustainable nutritional innovation for poultry.Poultry science · 2025Review
- Enhancing catalytic efficiency of Bacillus subtilis laccase BsCotA through active site pocket design.Applied microbiology and biotechnology · 2024Article
- Improving the Quality of Wheat Flour Bread by a Thermophilic Xylanase with Ultra Activity and Stability Reconstructed by Ancestral Sequence and Computational-Aided Analysis.Molecules (Basel, Switzerland) · 2024Article
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Authors and funding
8 authors.
Funding
Abstract
Laccases are widely used in industrial production due to their broad substrate availability and environmentally friendly nature. However, the pursuit of laccases with superior stability and increased heterogeneous expression to meet industry demands appears to be an ongoing challenge. To address this challenge, we resurrected five ancestral sequences of laccase BsCotA and their homologues. All five variants were successfully expressed in soluble and functional forms with improved expression levels in
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Registered trials
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