Evidence map›Paper›PMID 37443340›Full record

ArticleThe ISME journal2023

Trichlorobacter ammonificans, a dedicated acetate-dependent ammonifier with a novel module for dissimilatory nitrate reduction to ammonia.

Dimitry Y Sorokin, Tamara V Tikhonova, Hanna Koch, Eveline M van den Berg, Renske S Hinderks, Martin Pabst, Natalia I Dergousova, Anastasia Y Soloveva, Gijs J Kuenen, Vladimir O Popov and 2 more

Erratum issuedOpen access · bronzeAbstract read
In one paragraph

Article in The ISME journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 23 citations in OpenAlex.

  1. Aerobic dissimilatory nitrate reduction to ammonium inApplied and environmental microbiology · 2026
    Article
  2. Article
  3. Review
  4. Sulfonamide resistance geneMicrobiology spectrum · 2026
    Article
  5. Article
  6. Article
  7. Article
  8. Growth physiology, genomics, and proteomics ofFrontiers in microbiology · 2025
    Article
  9. Article
  10. Article
  11. "ISME communications · 2024
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 2 countries.

Dimitry Y SorokinDepartment of Biotechnology, Delft University of Technology, Delft, The Netherlands. d.sorokin@tudelft.nl.
Tamara V TikhonovaBach Institute of Biochemistry, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia.
Hanna KochDepartment of Microbiology, Radboud Institute for Biological and Environmental Sciences, Radboud University, Nijmegen, The Netherlands.
Eveline M van den BergDepartment of Biotechnology, Delft University of Technology, Delft, The Netherlands.
Renske S HinderksDepartment of Biotechnology, Delft University of Technology, Delft, The Netherlands.
Martin PabstDepartment of Biotechnology, Delft University of Technology, Delft, The Netherlands.ORCID http://orcid.org/0000-0001-9897-0723
Natalia I DergousovaBach Institute of Biochemistry, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia.ORCID http://orcid.org/0000-0002-4312-0825
Anastasia Y SolovevaBach Institute of Biochemistry, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia.
Gijs J KuenenDepartment of Biotechnology, Delft University of Technology, Delft, The Netherlands.
Vladimir O PopovBach Institute of Biochemistry, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia.
Mark C M van LoosdrechtDepartment of Biotechnology, Delft University of Technology, Delft, The Netherlands.ORCID http://orcid.org/0000-0003-0658-4775
Sebastian LückerDepartment of Microbiology, Radboud Institute for Biological and Environmental Sciences, Radboud University, Nijmegen, The Netherlands. s.luecker@science.ru.nl.ORCID http://orcid.org/0000-0003-2935-4454
Delft University of Technology · NLA N Bach Institute of Biochemistry · RURadboud University Nijmegen · NLRussian Academy of Sciences · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Dissimilatory nitrate reduction to ammonia (DNRA) is a common biochemical process in the nitrogen cycle in natural and man-made habitats, but its significance in wastewater treatment plants is not well understood. Several ammonifying Trichlorobacter strains (former Geobacter) were previously enriched from activated sludge in nitrate-limited chemostats with acetate as electron (e) donor, demonstrating their presence in these systems. Here, we isolated and characterized the new species Trichlorobacter ammonificans strain G1 using a combination of low redox potential and copper-depleted conditions. This allowed purification of this DNRA organism from competing denitrifiers. T. ammonificans is an extremely specialized ammonifier, actively growing only with acetate as e-donor and carbon source and nitrate as e-acceptor, but H

Indexed as

AmmoniaNitratesCytochromes cDenitrificationHumansNitrate ReductasesOxidation-ReductionAmmoniaCytochromes cNitrate ReductasesNitrates

Identifiers

PMID37443340
PMCPMC10504241
OpenAlexW4384204406

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.