ArticleScience (New York, N.Y.)2023
Multiplex CRISPR editing of wood for sustainable fiber production.
Article in Science (New York, N.Y.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
43 citing papers in PubMed.
- Strategies and mechanisms of precision genome engineering: From gene editing to genome writing.iMetaOmics · 2026Review
- Genetic transformation and CRISPR/Cas12a-mediated gene editing of European beech (Fagus sylvatica L.) employing a transient protoplast system.Communications biology · 2026Article
- Metabolic engineering for tobacco quality improvement: Advances and perspectives.Metabolic engineering communications · 2026Review
- Artificial Intelligence Methods in Forest Biotechnology: Current Status and Future Prospects.International journal of molecular sciences · 2026Review
- Targeting redundant gene families: A multiplexed, tissue-specific CRISPR toolbox for Arabidopsis genetic screens.Cell reports · 2026Article
- Sustainable bioenergy manufacturing in plants.Plant communications · 2026Review
- Advances in Double-Stranded DNA Targeting Technologies.Exploration (Beijing, China) · 2026Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Loss-of-function mutation of two phenylpropanoid pathway genes PAL1 and PAL2, coupled with MYB46 upregulation, increases fermentable sugar yield and ethanol production.Journal of biological engineering · 2025Article
- Streamlined multiplex CRISPR editing of receptor-like kinases in Populus via cell suspension transformation.Plant communications · 2025Article
- Woody Plant Transformation: Current Status, Challenges, and Future Perspectives.Plants (Basel, Switzerland) · 2025Review
- Conformational switch upon substrate binding informs the rational design of CCoAOMT enzymes.Scientific reports · 2025Article
- Advances in Genetic Engineering Techniques for Improved Forest Trees: Applications in Biomass, Stress Resilience and Carbon Sequestration.International journal of molecular sciences · 2025Review
- CRISPR/Cas9-germline editing ofScience advances · 2025Article
- Turbo-charging crop improvement: harnessing multiplex editing for polygenic trait engineering and beyond.The Plant journal : for cell and molecular biology · 2025Review
- The Role of MicroRNA-Based Strategies in Optimizing Plant Biomass Composition for Bio-Based Packaging Materials.Plants (Basel, Switzerland) · 2025Review
- Recent advances in breeding systems and their improvement in forage crops.Molecular biology reports · 2025Review
- An engineered CRISPR-Cas12i tool for efficient multiplexed genome editing.Nucleic acids research · 2025Article
- Factors underlying a latitudinal gradient in the S/G lignin monomer ratio in natural poplar variants.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- From Code to Life: The AI-Driven Revolution in Genome Editing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
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25 authors.
Funding
Abstract
The domestication of forest trees for a more sustainable fiber bioeconomy has long been hindered by the complexity and plasticity of lignin, a biopolymer in wood that is recalcitrant to chemical and enzymatic degradation. Here, we show that multiplex CRISPR editing enables precise woody feedstock design for combinatorial improvement of lignin composition and wood properties. By assessing every possible combination of 69,123 multigenic editing strategies for 21 lignin biosynthesis genes, we deduced seven different genome editing strategies targeting the concurrent alteration of up to six genes and produced 174 edited poplar variants. CRISPR editing increased the wood carbohydrate-to-lignin ratio up to 228% that of wild type, leading to more-efficient fiber pulping. The edited wood alleviates a major fiber-production bottleneck regardless of changes in tree growth rate and could bring unprecedented operational efficiencies, bioeconomic opportunities, and environmental benefits.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.