Evidence map›Paper›PMID 37440056›Full record

ArticleAdvances in experimental medicine and biology2023

Inhibition of Ryanodine Receptor 1 Reduces Endoplasmic Reticulum (ER) Stress and Promotes ER Protein Degradation in Cyclic Nucleotide-Gated Channel Deficiency.

Fan Yang, Hongwei Ma, Rekha Garg, Alfred Lewin, Xi-Qin Ding

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advances in experimental medicine and biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Fan YangDepartments of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
Hongwei MaDepartments of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
Rekha GargDepartment of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL, USA.
Alfred LewinDepartment of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL, USA.
Xi-Qin DingDepartments of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA. xi-qin-ding@ouhsc.edu.
University of Oklahoma Health Sciences Center · USUniversity of Florida · US

Funding

P30-CENTER CORE GRANT FOR VISION RESEARCHP30EY021725 · NEI · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI Michelle C Callegan, MICHAEL H ELLIOTT · 2011 to 2026
$10.5M
The Role of Endoplasmic Reticulum Calcium Channels in Cone Degeneration Resulting from CNG Channel DeficiencyR01EY027754 · NEI · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI DING, XI-QIN · 2017 to 2020
$1.5M
NEI NIH HHS P30 EY021725NEI NIH HHS R01 EY027754
6 · The paper itself

Abstract

The cone photoreceptor cyclic nucleotide-gated (CNG) channel plays a pivotal role in cone phototransduction. Mutations in genes encoding the channel subunits CNGA3 and CNGB3 account for about 80% of all cases of achromatopsia and are associated with progressive cone dystrophies. CNG channel deficiency leads to cellular/endoplasmic reticulum (ER) calcium dysregulation and ER stress-associated cone apoptosis. This work investigated the role of the ER calcium channel ryanodine receptor 1 (Ryr1) in ER stress and cone degeneration in CNG channel deficiency. The AAV-mediated CRISPR/SaCas9 genome editing was used to knock down Ryr1 specifically in cones. CNG channel-deficient mice displayed improved cone survival after subretinal injection of AAV2-SaCas9/gRNA-Ryr1, manifested as increased expression levels of cone proteins M-opsin, S-opsin, and cone arrestin. Knockdown of Ryr1 also led to reduced ER stress and increased expression levels of the ER-associated degradation proteins. This work demonstrates a role of Ryr1 in ER stress and cone degeneration in CNG channel deficiency, and supports strategies targeting ER calcium regulation for cone preservation.

Indexed as

Cyclic Nucleotide-Gated Cation ChannelsRyanodine Receptor Calcium Release ChannelAnimalsCalciumEndoplasmic ReticulumMiceNucleotides, CyclicOpsinsProteolysisRetinal Cone Photoreceptor CellsCalciumCyclic Nucleotide-Gated Cation ChannelsNucleotides, CyclicOpsinsRyanodine Receptor Calcium Release ChannelConeCyclic nucleotide-gated channelEndoplasmic reticulum-associated degradationEndoplasmic reticulum calcium homeostasisEndoplasmic reticulum stressRetinaRyanodine receptor

Identifiers

PMID37440056
PMCPMC11212467
OpenAlexW4384120803

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.