ArticleMethods in molecular biology (Clifton, N.J.)2023
Organic Extraction of Nucleic Acids Using Ethanol Precipitation or Microcon
Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Improved DNA recovery and STR profile development from weathered Bos taurus bones using demineralized bone slices.Journal of forensic sciences · 2025Article
- Development of a highly sensitive TaqMan method based on multi-probe strategy: its application in ASFV detection.Biology methods & protocols · 2024Article
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of organic solvents to separate nucleic acids from other cell components is a common practice among many scientific fields, including molecular biology and biochemistry. The advantage of performing organic extractions in forensic DNA analysis is the ability to purify DNA from heavily degraded or inhibitory sample types, such as skeletal remains. These sample types require special care to ensure that the DNA is contaminant-free since they often contain PCR inhibitors that negatively impact downstream DNA analysis, resulting in unobtainable or uninterpretable short tandem repeat (STR) profiles. Purification of DNA after an organic isolation procedure is essential for improving the likelihood of obtaining valid STR profile from a challenging evidence sample. This chapter describes the methodology for extracting and purifying DNA from various types of challenging samples that are often encountered in forensic casework.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.