Evidence map›Paper›PMID 37439173›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2023

[Expression of TUBB4B in mouse primary spermatocyte GC-2 cells and its regulatory effect on NF-κB and MAPK signaling pathway].

T Liu, W Wang, T Zhang, S Liu, Y Bian, C Zhang, R Xiao

Open access · greenAbstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

T LiuKey Laboratory of Molecular Pathology of Inner Mongolia Autonomous Region, Inner Mongolia Medical University, Hohhot 010059, China.
W WangKey Laboratory of Molecular Pathology of Inner Mongolia Autonomous Region, Inner Mongolia Medical University, Hohhot 010059, China.
T ZhangKey Laboratory of Molecular Pathology of Inner Mongolia Autonomous Region, Inner Mongolia Medical University, Hohhot 010059, China.
S LiuKey Laboratory of Molecular Pathology of Inner Mongolia Autonomous Region, Inner Mongolia Medical University, Hohhot 010059, China.
Y BianKey Laboratory of Molecular Pathology of Inner Mongolia Autonomous Region, Inner Mongolia Medical University, Hohhot 010059, China.
C ZhangDepartment of Pharmacy, Inner Mongolia Medical University, Hohhot 010059, China.
R XiaoKey Laboratory of Molecular Pathology of Inner Mongolia Autonomous Region, Inner Mongolia Medical University, Hohhot 010059, China.
Inner Mongolia Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo explore the interaction between Tubulin beta 4B class IVb (TUBB4B) and Agtpbp1/cytosolic carboxypeptidase- like1 (CCP1) in mouse primary spermatocytes (GC-2 cells) and the role of TUBB4B in regulating the development of GC-2 cells.

methodsLentiviral vectors were used to infect GC-2 cells to construct TUBB4B knockdown and negative control (NC-KD) cells. The stable cell lines with TUBB4B overexpression (Tubb4b-OE) and the negative control (NC-OE) cells were screened using purinomycin. RT-qPCR and Western blotting were used to verify successful cell modeling and explore the relationship between TUBB4B and CCP1 expressions in GC-2 cells. The effects of TUBB4B silencing and overexpression on the proliferation and cell cycle of GC-2 cells were evaluated using CCK8 assay and flow cytometry. The signaling pathway proteins showing significant changes in response to TUBB4B silencing or overexpression were identified using Western blotting and immunofluorescence assay and then labeled for verification at the cellular level.

resultsBoth TUBB4B silencing and overexpression in GC-2 cells caused consistent changes in the mRNA and protein expressions of CCP1 (

conclusionsTUBB4B and CCP1 interact via a mutual positive regulation mechanism in GC-2 cells. CCP-1 can deglutamize TUBB4B, and the latter is involved in the regulation of NF-κB and MAPK signaling pathways in primary spermatocytes.

Indexed as

NF-kappa BSerine-Type D-Ala-D-Ala CarboxypeptidaseSpermatocytesTubulinAnimalsGTP-Binding ProteinsMaleMiceMitogen-Activated Protein KinasesRNA, MessengerSignal TransductionAgtpbp1 protein, mouseGTP-Binding ProteinsMitogen-Activated Protein KinasesNF-kappa BRNA, MessengerSerine-Type D-Ala-D-Ala CarboxypeptidaseTubulincytosolic carboxypeptidase-like1male sterilitymitogen-activated protein kinase MAPK pathwaynuclear factor kappa-B pathwayprimary spermatocytesTUBB4B

Identifiers

PMID37439173
PMCPMC10339298
OpenAlexW4384120668

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.