Evidence map›Paper›PMID 37438396›Full record

ArticleScientific reports2023

Hydrogen peroxide from L-amino acid oxidase of king cobra (Ophiophagus hannah) venom attenuates Pseudomonas biofilms.

Uthaibhorn Singkham-In, Wichit Thaveekarn, Jureeporn Noiphrom, Orawan Khow, Surada Ponwaranon, Jiraphorn Issara-Amphorn, Visith Sitprija, Asada Leelahavanichkul

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.3field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Uthaibhorn Singkham-InDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, 1873 Rama 4 Road, Pathumwan, Bangkok, 10330, Thailand.
Wichit ThaveekarnQueen Saovabha Memorial Institute, Thai Red Cross Society, 1871 Rama 4 Road, Pathumwan, Bangkok, 10330, Thailand.
Jureeporn NoiphromQueen Saovabha Memorial Institute, Thai Red Cross Society, 1871 Rama 4 Road, Pathumwan, Bangkok, 10330, Thailand.
Orawan KhowQueen Saovabha Memorial Institute, Thai Red Cross Society, 1871 Rama 4 Road, Pathumwan, Bangkok, 10330, Thailand.
Surada PonwaranonDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, 1873 Rama 4 Road, Pathumwan, Bangkok, 10330, Thailand.
Jiraphorn Issara-AmphornFunctional Cellular Networks Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, 20892, USA.
Visith SitprijaQueen Saovabha Memorial Institute, Thai Red Cross Society, 1871 Rama 4 Road, Pathumwan, Bangkok, 10330, Thailand.
Asada LeelahavanichkulDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, 1873 Rama 4 Road, Pathumwan, Bangkok, 10330, Thailand. aleelahavanit@gmail.com.
Chulalongkorn University · THQueen Saovabha Memorial Institute · THThai Red Cross Society · THNational Institute of Allergy and Infectious Diseases · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Because of the high incidence of Pseudomonas aeruginosa biofilms-related nosocomial infections, venoms from common Thai snakes were tested. Although venoms from king cobra (Ophiophagus hannah; OH) and green pit viper (Trimeresurus albolabris) showed the broadest antibacterial spectrum, OH venom demonstrated more profound anti-biofilm activities against P. aeruginosa. Additionally, purified L-amino acid oxidase from OH venom (OH-LAAO), using a three-step chromatography and protein identification, reduced biofilm mass as indicated by the downregulation of several genes, including the genes for biofilm synthesis (algD and pslB) and biofilm regulators (algU, gacA, and siaD). Moreover, OH-LAAO disrupted Pseudomonas-preformed biofilms via upregulation of several genes for biofilm dispersion (nbdA, bdlA, and dipA) and biofilm degradation (endA and pslG), resulting in a reduction of the biofilm biomass. Due to the antimicrobial effects and anti-biofilm activities (reduced production plus increased dispersion) neutralized by catalase, a hydrogen peroxide (H

Indexed as

PseudomonasPseudomonas InfectionsAnimalsHydrogen PeroxideL-Amino Acid OxidaseOphiophagus hannahPseudomonas aeruginosaHydrogen PeroxideL-Amino Acid Oxidase

Identifiers

PMID37438396
PMCPMC10338509
OpenAlexW4384008724

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.