Evidence map›Paper›PMID 37436554›Full record

ArticleVeterinary research communications2023

Molecular characterization and function of JAK/STAT pathway in IPEC-J2 cells during Clostridium perfringens beta2 toxin stimulation.

Xiaoli Gao, Pengfei Wang, Zunqiang Yan, Qiaoli Yang, Xiaoyu Huang, Shengwei Zhang, Shuangbao Gun

Abstract read
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Article in Veterinary research communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 86% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Xiaoli GaoCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, China.ORCID http://orcid.org/0000-0003-4908-1051
Pengfei WangCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, China.
Zunqiang YanCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, China.
Qiaoli YangCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, China.
Xiaoyu HuangCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, China.
Shengwei ZhangFarmer Education and Training Work Station of Gansu province, Lanzhou, 730070, China.
Shuangbao GunCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, China. gunsbao056@126.com.
Gansu Agricultural University · CN

Funding

Scientific Research Start-Up Funds for Openly-Recruited Doctors of Gansu Agricultural University GAU-KYQD-2021-04
6 · The paper itself

Abstract

Intestinal infection with C. perfringens is responsible for outbreaks of diarrhea in piglets. Janus kinase / signal transducer and activator of transcription (JAK/STAT) is a vital signaling pathway that regulates cellular activity and inflammatory response, closely correlated with multiple diseases development and advances. Currently, the potential effect of JAK/STAT on C. perfringens beta2 (CPB2) treatment on porcine intestinal epithelial (IPEC-J2) cells has not been explored. The expression of JAK/STAT genes or proteins in IPEC-J2 cells induced by CPB2 were observed by qRT-PCR and Western blot, and further used WP1066 to explore the effect of JAK2/STAT3 on mechanism employed by CPB2 on apoptosis, cytotoxicity, oxidative stress and inflammatory cytokines of IPEC-J2 cells. JAK2, JAK3, STAT1, STAT3, STAT5A and STAT6 were highly expressed in CPB2-induced IPEC-J2 cells, among which STAT3 had the highest expression. Moreover, apoptosis, cytotoxicity and oxidative stress were attenuated via blocking the activation of JAK2/STAT3 by using WP1066 in CPB2-treated IPEC-J2 cells. Furthermore, WP1066 significantly suppressed the secretion of interleukin (IL)-6, IL-1β and TNF-α induced by CPB2 in IPEC-J2 cells.Our findings provide some insights into the functional roles of JAK2/STAT3 in piglets against to C. perfringens infection.

Indexed as

Clostridium InfectionsClostridium perfringensSignal TransductionSwine DiseasesAnimalsBacterial ToxinsBlotting, WesternCell LineGene Expression ProfilingIntestinesJanus KinasesPyridinesReal-Time Polymerase Chain ReactionSwineTyrphostinsBacterial Toxinscpb2 protein, Clostridium perfringensJanus KinasesPyridinesTyrphostinsWP1066ApoptosisC. perfringens beta2Inflammatory responseJAK2/STAT3 pathwayPig

Identifiers

PMID37436554
OpenAlexW4383998165

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.