Integration of Lysin into Chitosan Nanoparticles for Improving Bacterial Biofilm Inhibition.

Bingxin Liu, Zong Li, Qiucui Guo, Xiaoxiao Guo, Ruyin Liu, Xinchun Liu

Abstract read

In one paragraph

Article in Applied biochemistry and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it

0cells of the map it votes in

2citing papers in PubMed

0.5field-weighted citation impact, top 31% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

Designing the Next Generation of Endolysins: A Triple Strategy Integrating Bioinformatics Mining, Engineering Modification and Encapsulation Formulation.Probiotics and antimicrobial proteins · 2026
Review
Bacteriophage lysins: a promising strategy for combating foodborne pathogens in food safety.Frontiers in microbiology · 2026
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Bingxin LiuCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China.

Zong LiCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China.

Qiucui GuoCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China.

Xiaoxiao GuoCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China.

Ruyin LiuCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China. Lry1981@ucas.ac.cn.

Xinchun LiuCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China. xcliu@ucas.ac.cn.ORCID http://orcid.org/0000-0003-3834-6333

University of Chinese Academy of Sciences · CN

Funding

Fundamental Research Funds for the Central Universities E1E40506National Key Laboratory of Science, Technology on Advanced Composites in Special Environments, HIT GYY-DTFZ-2022-008

6 · The paper itself

Abstract

Bacterial biofilms (BBFs) exhibit high drug resistance, antiphagocytosis, and extremely strong adhesion, and therefore can cause various diseases. They are also one of the important causes of bacterial infections. Thus, the effective removal of BBFs has attracted considerable research interest. Endolysins, which are efficient antibacterial bioactive macromolecules, have recently been receiving increasing attention. In this study, we overcame the deficiencies of endolysins via immobilization on chitosan nanoparticles (CS-NPs) by preparing LysST-3-CS-NPs using the ionic cross-linking reaction between CS-NPs and LysST-3, an endolysin purified using phage ST-3 expression. The obtained LysST-3-CS-NPs were verified and thoroughly characterized, their antimicrobial activity was investigated using microscopy, and their antibacterial efficacy on polystyrene surfaces was studied. The results obtained suggested that LysST-3-CS-NPs exhibit enhanced bactericidal properties and increased stability and can serve as reliable biocontrol agents for the prevention and treatment of Salmonella biofilm infections.

Indexed as

ChitosanNanoparticlesAnti-Bacterial AgentsBacteriaBiofilmsAnti-Bacterial AgentsChitosanBacterial biofilmsBacteriophage endolysinChitosan nanoparticlesGram-positive bacteriaSalmonella

Identifiers

PMID37436548

DOI10.1002/med.21542

OpenAlexW4383999392

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.