Evidence map›Paper›PMID 37435613›Full record

ArticleKorean journal of anesthesiology2023

Effects of sevoflurane on metalloproteinase and natural killer group 2, member D (NKG2D) ligand expression and natural killer cell-mediated cytotoxicity in breast cancer: an in vitro study.

Hyae Jin Kim, Soeun Jeon, Hyeon Jeong Lee, Jaeho Bae, Hyun-Su Ri, Jeong-Min Hong, Sung In Paek, Seul Ki Kwon, Jae-Rin Kim, Seungbin Park and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Korean journal of anesthesiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 1 country.

Hyae Jin KimDepartment of Anesthesia and Pain Medicine, Pusan National University School of Medicine, Busan, Korea.
Soeun JeonDepartment of Anesthesiology and Pain Medicine, School of Dentistry, Institute for Translational Research in Dentistry, Kyungpook National University, Daegu, Korea.
Hyeon Jeong LeeDepartment of Anesthesia and Pain Medicine, Pusan National University School of Medicine, Busan, Korea.
Jaeho BaeDepartment of Biochemistry, Pusan National University School of Medicine, Yangsan, Korea.
Hyun-Su RiDepartment of Anesthesiology and Pain Medicine, Kyungpook National University School of Medicine, Daegu, Korea.
Jeong-Min HongDepartment of Anesthesia and Pain Medicine, Pusan National University School of Medicine, Busan, Korea.
Sung In PaekDepartment of Anesthesia and Pain Medicine, Pusan National University School of Medicine, Busan, Korea.
Seul Ki KwonDepartment of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.
Jae-Rin KimDepartment of Anesthesia and Pain Medicine, Pusan National University School of Medicine, Busan, Korea.
Seungbin ParkBiomedical Research Institute, Pusan National University Hospital, Busan, Korea.
Eun-Jung YunDepartment of Biochemistry, Pusan National University School of Medicine, Yangsan, Korea.
Pusan National University · KRPusan National University Hospital · KRKyungpook National University · KRSeoul National University · KR

Funding

Pusan National University Hospital 20210033
6 · The paper itself

Abstract

backgroundWe investigated the effects of sevoflurane exposure on the expression of matrix metalloproteinase (MMP), expression and ablation of natural killer group 2, member D (NKG2D) ligands (UL16-binding proteins 1-3 and major histocompatibility complex class I chain-related molecules A/B), and natural killer (NK) cell-mediated cytotoxicity in breast cancer cells.

methodsThree human breast cancer cell lines (MCF-7, MDA-MB-453, and HCC-70) were incubated with 0 (control), 600 (S6), or 1200 μM (S12) sevoflurane for 4 h. The gene expression of NKG2D ligands and their protein expression on cancer cell surfaces were measured using multiplex polymerase chain reaction (PCR) and flow cytometry, respectively. Protein expression of MMP-1 and -2 and the concentration of soluble NKG2D ligands were analyzed using western blotting and enzyme-linked immunosorbent assays, respectively.

resultsSevoflurane downregulated the mRNA and protein expression of the NKG2D ligand in a dose-dependent manner in MCF-7, MDA-MB-453, and HCC-70 cells but did not affect the expression of MMP-1 or -2 or the concentration of soluble NKG2D ligands in the MCF-7, MDA-MB-453, and HCC-70 cells. Sevoflurane attenuated NK cell-mediated cancer cell lysis in a dose-dependent manner in MCF-7, MDA-MB-453, and HCC-70 cells (P = 0.040, P = 0.040, and P = 0.040, respectively).

conclusionsOur results demonstrate that sevoflurane exposure attenuates NK cell-mediated cytotoxicity in breast cancer cells in a dose-dependent manner. This could be attributed to a sevoflurane-induced decrease in the transcription of NKG2D ligands rather than sevoflurane-induced changes in MMP expression and their proteolytic activity.

Indexed as

Breast NeoplasmsCarcinoma, HepatocellularLiver NeoplasmsFemaleHumansKiller Cells, NaturalLigandsMatrix Metalloproteinase 1NK Cell Lectin-Like Receptor Subfamily KSevofluraneLigandsMatrix Metalloproteinase 1NK Cell Lectin-Like Receptor Subfamily KSevofluraneBreast neoplasmsInhalation anestheticsMatrix metalloproteinasesNatural killer cellsSevofluraneTumor escape.

Identifiers

PMID37435613
PMCPMC10718625
OpenAlexW4383999382

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.