Evidence map›Paper›PMID 37435391›Full record

ArticleJournal of biomolecular techniques : JBT2023

2019 Association of Biomolecular Resource Facilities Multi-Laboratory Data-Independent Acquisition Proteomics Study.

Joanna Kirkpatrick, Paul M Stemmer, Brian C Searle, Laura E Herring, LeRoy Martin, Mukul K Midha, Brett S Phinney, Baozhen Shan, Magnus Palmblad, Yan Wang and 2 more

Abstract read
In one paragraph

Article in Journal of biomolecular techniques : JBT, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Joanna KirkpatrickLeibniz Institute on Aging Fritz Lipmann Institute 07745Jena Germany.ORCID 0000-0001-9291-7294
Paul M StemmerWayne State University DetroitMichigan48202 USA.ORCID 0000-0003-3315-9846
Brian C SearleDepartment of Biomedical Informatics The Ohio State University ColumbusOhio43210 USA.ORCID 0000-0001-8760-6731
Laura E HerringUNC Proteomics Core Facility Department of Pharmacology University of North Carolina at Chapel Hill Chapel HillNorth Carolina27514 USA.ORCID 0000-0003-4496-7312
LeRoy MartinWaters Corporation BeverlyMassachusetts01915 USA.ORCID 0000-0002-7161-1541
Mukul K MidhaInstitute for Systems Biology SeattleWashington98109 USA.ORCID 0000-0003-4053-0682
Brett S PhinneyUniversity of California, Davis DavisCalifornia95616 USA.ORCID 0000-0003-3870-3302
Baozhen ShanBioinformatics Solutions Inc. WaterlooON N2L 3K8 Canada.ORCID 0000-0002-8757-5493
Magnus PalmbladCenter for Proteomics and Metabolomics Leiden University Medical Center 2333 ZC Leiden The Netherlands.ORCID 0000-0002-5865-8994
Yan WangNational Institute of Dental and Craniofacial Research National Institutes of Health BethesdaMaryland20892 USA.ORCID 0000-0003-1187-9527
Pratik D JagtapDepartment of Biochemistry Molecular Biology and Biophysics University of Minnesota MinneapolisMinnesota55455 USA.ORCID 0000-0003-0984-0973
Benjamin A NeelyNational Institute of Standards and Technology CharlestonSouth Carolina29412 USA.ORCID 0000-0001-6120-7695

Funding

TRANSLATIONAL RESEARCHP30CA076292 · NCI · UNIVERSITY OF SOUTH FLORIDA · PI KENNETH Lynn WRIGHT · 1998 to 2026
$93.5M
Mass Spectrometry FacilityZICDE000751 · NIDCR · NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH · PI WANG, YAN · 2020 to 2025
$3.6M
Expanding multiplexed kinase biosensor analysis to SWATH-MS (Part 2 of 2)U24CA199347 · NCI · UNIVERSITY OF MINNESOTA · PI GRIFFIN, TIMOTHY J. · 2016 to 2019
$2.4M
QE-HF-X Mass SpectrometerS10OD026918 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI PHINNEY, BRETT S · 2020 to 2020
$600k
Cancer Research UK FC001999Medical Research Council FC001999NCI NIH HHS P30 CA076292NCI NIH HHS U24 CA199347NIH HHS S10 OD026918Wellcome Trust FC001999
6 · The paper itself

Abstract

Despite the advantages of fewer missing values by collecting fragment ion data on all analytes in the sample as well as the potential for deeper coverage, the adoption of data-independent acquisition (DIA) in proteomics core facility settings has been slow. The Association of Biomolecular Resource Facilities conducted a large interlaboratory study to evaluate DIA performance in proteomics laboratories with various instrumentation. Participants were supplied with generic methods and a uniform set of test samples. The resulting 49 DIA datasets act as benchmarks and have utility in education and tool development. The sample set consisted of a tryptic HeLa digest spiked with high or low levels of 4 exogenous proteins. Data are available in MassIVE MSV000086479. Additionally, we demonstrate how the data can be analyzed by focusing on 2 datasets using different library approaches and show the utility of select summary statistics. These data can be used by DIA newcomers, software developers, or DIA experts evaluating performance with different platforms, acquisition settings, and skill levels.

Indexed as

BenchmarkingProteomicsDrugs, GenericEducational StatusGene LibraryHumansDrugs, Genericdata-independent acquisitionlabel-free quantificationproteomicsspike-in quantification

Identifiers

PMID37435391
PMCPMC10332336

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.