ArticleThe Plant cell2024
Imaging of plant calcium-sensor kinase conformation monitors real time calcium-dependent decoding in planta.
Article in The Plant cell, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Review
- An atypical EF-hand domain containing resistance protein SRC4 boosts plant immunity in a calcium-enhanced manner.The Plant journal : for cell and molecular biology · 2026Article
- Phosphorylation networks as regulatory hubs in plant stress signaling: kinase dynamics, crosstalk, and network plasticity.Plant cell reports · 2026Review
- JrCDPK13L-mediated phosphorylation of JrERF113L promotes walnut resistance to Colletotrichum gloeosporioides.Plant physiology · 2026Article
- The architecture of salt tolerance: A multi-scale view of sodium transport in plants.Plant communications · 2026Review
- Calcium-Dependent Protein Kinases in Plants: Structure, Signaling, and Multifaceted Regulatory Roles in Development and Stress Adaptation.International journal of molecular sciences · 2026Review
- Calcium signaling in crops.The New phytologist · 2026Review
- Role of AtCPK5 and AtCPK6 in the regulation of the plant immune response triggered by rhamnolipids in Arabidopsis.PloS one · 2026Article
- Loss of calcium-dependent protein kinases OsCPK5 and OsCPK13 leads to NLR-dependent resistance in rice.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Differential regulation of calcium-activated plant kinases in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2025Article
- CaEMBO reports · 2025Article
- Genome-Wide Investigation ofInternational journal of molecular sciences · 2025Article
- ABA-activated low-nanomolar CaNature plants · 2025Article
- Phosphatases: Decoding the Role of Mycorrhizal Fungi in Plant Disease Resistance.International journal of molecular sciences · 2024Review
- Calcium imaging: a technique to monitor calcium dynamics in biological systems.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Changes in cytosolic calcium (Ca2+) concentration are among the earliest reactions to a multitude of stress cues. While a plethora of Ca2+-permeable channels may generate distinct Ca2+ signatures and contribute to response specificities, the mechanisms by which Ca2+ signatures are decoded are poorly understood. Here, we developed a genetically encoded Förster resonance energy transfer (FRET)-based reporter that visualizes the conformational changes in Ca2+-dependent protein kinases (CDPKs/CPKs). We focused on two CDPKs with distinct Ca2+-sensitivities, highly Ca2+-sensitive Arabidopsis (Arabidopsis thaliana) AtCPK21 and rather Ca2+-insensitive AtCPK23, to report conformational changes accompanying kinase activation. In tobacco (Nicotiana tabacum) pollen tubes, which naturally display coordinated spatial and temporal Ca2+ fluctuations, CPK21-FRET, but not CPK23-FRET, reported oscillatory emission ratio changes mirroring cytosolic Ca2+ changes, pointing to the isoform-specific Ca2+-sensitivity and reversibility of the conformational change. In Arabidopsis guard cells, CPK21-FRET-monitored conformational dynamics suggest that CPK21 serves as a decoder of signal-specific Ca2+ signatures in response to abscisic acid and the flagellin peptide flg22. Based on these data, CDPK-FRET is a powerful approach for tackling real-time live-cell Ca2+ decoding in a multitude of plant developmental and stress responses.
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Registered trials
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