ArticleJournal of assisted reproduction and genetics2023
Should embryo rebiopsy be considered a regular strategy to increase the number of embryos available for transfer?
Article in Journal of assisted reproduction and genetics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 4 of them syntheses that pooled it.
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Who cites it
13 citing papers in PubMed, 4 syntheses or guidelines pooled it.
- Impact of double versus single blastocyst biopsy and vitrification on clinical and neonatal outcomes in PGT cycles: A systematic review and meta-analysis of embryo retesting.Acta obstetricia et gynecologica Scandinavica · 2026Pooled it
- Does double cryopreservation as well as double biopsy affect embryo viability and clinical outcomes? Evidence from a systematic review of the literature.Journal of assisted reproduction and genetics · 2025Pooled it
- Embryonic and neonatal outcomes following double vitrification/thawing: a systematic review and meta-analysis.BMC pregnancy and childbirth · 2025Pooled it
- Impacts of double biopsy and double vitrification on the clinical outcomes following euploid blastocyst transfer: a systematic review and meta-analysis.Human reproduction (Oxford, England) · 2024Pooled it
- PGT-A reveals triploid embryos among normally fertilized oocytes: frequency and blastocyst features.Journal of assisted reproduction and genetics · 2026Article
- Impact of repeated cryopreservation on embryo development and chromosomal ploidy.Archives of gynecology and obstetrics · 2026Article
- Genetic analysis of biopsy-related droplets in patients undergoing PGT-A and its potential application.Frontiers in endocrinology · 2026Article
- Automated AI for real-time sperm selection in ICSI: reducing variability and studying the role of sperm in embryo development.Reproductive biology and endocrinology : RB&E · 2025Observational
- Impact of PGT Introduction on IVF Laboratory Workload: Lessons Learned from a Single-Center Experience of 5258 Biopsies over a 10-Year Period.Life (Basel, Switzerland) · 2025Article
- Case report: re-biopsy rescues a to-be-discarded segmental aneuploid embryo resulting in a healthy pregnancy with normal prenatal testing results.Journal of assisted reproduction and genetics · 2025Article
- Double versus single blastocyst biopsy and vitrification in preimplantation genetic testing (PGT) cycles: protocol for a systematic review and meta-analysis of clinical and neonatal outcomes.Systematic reviews · 2025Article
- Trophectoderm Biopsy: Present State of the Art.Genes · 2025Review
- The impact of a second embryo biopsy for preimplantation genetic testing for monogenic diseases (PGT-M) with inconclusive results on pregnancy potential: results from a matched case-control study.Journal of assisted reproduction and genetics · 2024Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo investigate whether embryo rebiopsy increases the yield of in vitro fertilization (IVF) cycles.
methodsRetrospective study including 18,028 blastocysts submitted for trophectoderm biopsy and preimplantation genetic testing for aneuploidy (PGT-A) between January 2016 and December 2021 in a private IVF center. Out of the 517 embryos categorized as inconclusive, 400 survived intact to the warming procedure, re-expanded, and were suitable for rebiopsy. Of them, 71 rebiopsied blastocysts were transferred. Factors affecting the probability of obtaining an undiagnosed blastocyst and clinical outcomes from blastocysts biopsied once and twice were investigated.
resultsThe overall diagnostic rate was 97.1%, with 517 blastocysts receiving inconclusive reports. Several blastocyst and laboratory features, such as the day of the biopsy, the stage of development, and the biopsy methodology, were related to the risk of obtaining an inconclusive diagnosis after PGT-A. A successful diagnosis was obtained in 384 of the rebiopsied blastocysts, 238 of which were chromosomally transferable. A total of 71 rebiopsied blastocysts were transferred, resulting in 32 clinical pregnancies [(clinical pregnancy rate (CPR)=45.1%], 16 miscarriages [(miscarriage rate (MR)=41%], and, until September 2020, 12 live births [(live birth rate (LBR)=23.1%]. A significantly lower LBR and higher MR were obtained after transferring rebiopsied blastocysts compared to those biopsied once.
conclusionAlthough an extra round of biopsy and vitrification may cause a detrimental effect on embryo viability, re-analyzing the test-failure blastocysts contributes to increasing the number of euploid blastocysts available for transfer and the LBR.
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