ArticleThe EMBO journal2023
Characterization of the REC114-MEI4-IHO1 complex regulating meiotic DNA double-strand break formation.
Article in The EMBO journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 20 citations in OpenAlex.
- Controlling Meiotic Double-Strand Break Formation in Mice: A Web of Multivalent Protein-Protein Interactions.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- Stepwise pairing and fast transition regulated by recombination drive meiotic chromosome interactions.Nature communications · 2026Article
- Multifaceted and evolutionarily dynamic interactions betweenbioRxiv : the preprint server for biology · 2026Article
- Structure and function of the synaptonemal complex.The Journal of cell biology · 2026Review
- Mei4 deficiency induces sexual dimorphism in early meiosis and promotes production of unreduced eggs in zebrafish.Zoological research · 2026Article
- Genetic Mutations and Non-Genomic Dysregulation in Human Preimplantation Embryo Arrest.International journal of molecular sciences · 2026Review
- Article
- Genetic and functional analysis reveals novel mutations in meiotic genes underlying non-obstructive azoospermia.Journal of assisted reproduction and genetics · 2025Article
- Exome sequencing identifies genes for socioeconomic status in 350,770 individuals.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Identification of prognostic hub genes and functional role of BAIAP2L2 in prostate cancer progression: a transcriptomic and experimental study.Frontiers in immunology · 2025Article
- Cryo-EM structures of the Spo11 core complex bound to DNA.Nature structural & molecular biology · 2025Article
- The TOPOVIBL meiotic DSB formation protein: new insights from its biochemical and structural characterization.Nucleic acids research · 2024Article
- Seeding the meiotic DNA break machinery and initiating recombination on chromosome axes.Nature communications · 2024Article
- The molecular machinery of meiotic recombination.Biochemical Society transactions · 2024Review
- Cryo-EM structure of the Spo11 core complex bound to DNA.bioRxiv : the preprint server for biology · 2023Article
- Characterization of the REC114-MEI4-IHO1 complex regulating meiotic DNA double-strand break formation.The EMBO journal · 2023Article
- Evolutionary conservation of the structure and function of meiotic Rec114-Mei4 and Mer2 complexes.Genes & development · 2023Article
- Structure and DNA-bridging activity of the essential Rec114-Mei4 trimer interface.Genes & development · 2023Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Meiotic recombination is initiated by the formation of DNA double-strand breaks (DSBs), essential for fertility and genetic diversity. In the mouse, DSBs are formed by the catalytic TOPOVIL complex consisting of SPO11 and TOPOVIBL. To preserve genome integrity, the activity of the TOPOVIL complex is finely controlled by several meiotic factors including REC114, MEI4, and IHO1, but the underlying mechanism is poorly understood. Here, we report that mouse REC114 forms homodimers, that it associates with MEI4 as a 2:1 heterotrimer that further dimerizes, and that IHO1 forms coiled-coil-based tetramers. Using AlphaFold2 modeling combined with biochemical characterization, we uncovered the molecular details of these assemblies. Finally, we show that IHO1 directly interacts with the PH domain of REC114 by recognizing the same surface as TOPOVIBL and another meiotic factor ANKRD31. These results provide strong evidence for the existence of a ternary IHO1-REC114-MEI4 complex and suggest that REC114 could act as a potential regulatory platform mediating mutually exclusive interactions with several partners.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.