ArticleFoods (Basel, Switzerland)2022
A Droplet Digital PCR Based Approach for Identification and Quantification of Porcine and Chicken Derivatives in Beef.
Article in Foods (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- A ddPCR method for simultaneous detection and quantification of Salmonella enterica, Staphylococcus aureus, Listeria monocytogenes, and Bacillus cereus in foods.Scientific reports · 2025Article
- Molecular methods for rapid detection and identification of foodborne pathogenic bacteria.World journal of microbiology & biotechnology · 2025Review
- A Droplet Digital PCR-Based Approach for Quantitative Analysis of the Adulteration of Atlantic Salmon with Rainbow Trout.Foods (Basel, Switzerland) · 2023Article
- Quantification of Pork, Chicken, Beef, and Sheep Contents in Meat Products Using Duplex Real-Time PCR.Foods (Basel, Switzerland) · 2023Article
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Authors and funding
6 authors.
Funding
Abstract
Adulteration of high-value beef with lower-priced alternatives is a world-wide problem resulting in consumers' distrust and market chaos. Therefore, effective methods for the identification and quantification of adulterated beef products are urgently needed. In this study, we developed a reliable droplet digital PCR (ddPCR) method targeting the single-copy nuclear genes for qualitative and quantitative detection of the presence of porcine and chicken derivatives in beef. A fixed constant (transfer coefficient) was introduced to directly transform the ratio of DNA copy number to the mass proportion of targeted meats. Results revealed that the linearity range of quantification for pork and chicken were both from 1% (
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