Evidence map›Paper›PMID 37430045›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Optical pH Monitoring in Microdroplet Platforms for Live Cell Experiments Using Colloidal Surfactants.

Xuyan Lin, Wenting Qiu, Steevanson Bayer, Stefan Nagl

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Xuyan LinDepartment of Chemistry, Hong Kong University of Science and Technology, Kowloon, Hong Kong, China.
Wenting QiuDepartment of Chemistry, Hong Kong University of Science and Technology, Kowloon, Hong Kong, China.
Steevanson BayerDepartment of Chemistry, Hong Kong University of Science and Technology, Kowloon, Hong Kong, China.
Stefan NaglDepartment of Chemistry, Hong Kong University of Science and Technology, Kowloon, Hong Kong, China. chnagl@ust.hk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Droplet microfluidic technology facilitates the development of high-throughput screening applications in nanoliter volumes. Surfactants provide stability for emulsified monodisperse droplets to carry out compartmentalization. Fluorinated silica-based nanoparticles are used; they can minimize crosstalk in microdroplets and provide further functionalities by surface labeling. Here we describe a protocol for monitoring pH changes in live single cells by fluorinated silica nanoparticles, for their synthesis, chip fabrication, and optical monitoring on the microscale. The nanoparticles are doped with ruthenium-tris-1,10-phenanthroline dichloride on the inside and conjugated with fluorescein isothiocyanate on the surface. This protocol may be used more generally to detect pH changes in microdroplets. The fluorinated silica nanoparticles can also be used as droplet stabilizers with an integrated luminescent sensor for other applications.

Indexed as

High-Throughput Screening AssaysSilicon DioxideCross ReactionsFluoresceinHydrogen-Ion ConcentrationFluoresceinSilicon DioxideDroplet microfluidicsFluorinated surfactantsLuminescence lifetime imagingLuminescent pH sensorSilica nanoparticles

Identifiers

PMID37430045

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.