ArticleNature methods2023
Dense 4D nanoscale reconstruction of living brain tissue.
Article in Nature methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- Tidying up white matter: Neuroplastic transformations in sensorimotor tracts following slackline skill acquisition.Human brain mapping · 2024Trial
- PRMix: Primary Region Mix Augmentation and Benchmark Dataset for Precise Whole Mouse Brain Anatomical Delineation.NeuroImage · 2026Article
- 3D STED Super-Resolution Imaging Strategy for Visualizing Synaptic Nano-architecture in Brain Cryosections.Bio-protocol · 2026Article
- Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo.Nature methods · 2026Article
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- A general one-step protocol to generate impermeable fluorescent HaloTag substrates for in situ live cell application and super-resolution imaging.Nature communications · 2026Article
- Grand challenges for systems neuroscience: perspectives and opportunities.Frontiers in systems neuroscience · 2026Article
- 3D Quantification of Viral Transduction Efficiency in Living Human Retinal Organoids.Small methods · 2026Article
- Topology-driven energy transfer networks for upconversion stimulated emission depletion microscopy.Light, science & applications · 2025Article
- Smart 3D super-resolution microscopy reveals the architecture of the RNA scaffold in a nuclear body.Nature communications · 2025Article
- Nanoscale Imaging of Biological Tissues: Techniques, Challenges and Emerging Frontiers.Nanomaterials (Basel, Switzerland) · 2025Review
- Review
- Live STED imaging of functional neuroanatomy.Nature protocols · 2025Review
- Article
- Live imaging of the extracellular matrix with a glycan-binding fluorophore.Nature methods · 2025Article
- Combining nanobody labeling with STED microscopy reveals input-specific and layer-specific organization of neocortical synapses.PLoS biology · 2025Article
- 3D Super-Resolution Imaging of PSD95 Reveals an Abundance of Diffuse Protein Supercomplexes in the Mouse Brain.ACS chemical neuroscience · 2025Article
- Imaging brain tissue architecture across millimeter to nanometer scales.Nature biotechnology · 2024Article
- Imaging the extracellular matrix in live tissues and organisms with a glycan-binding fluorophore.bioRxiv : the preprint server for biology · 2024Article
- Year in review 2023.Nature methods · 2024Article
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Authors and funding
20 authors at 6 institutions in 4 countries.
Funding
Abstract
Three-dimensional (3D) reconstruction of living brain tissue down to an individual synapse level would create opportunities for decoding the dynamics and structure-function relationships of the brain's complex and dense information processing network; however, this has been hindered by insufficient 3D resolution, inadequate signal-to-noise ratio and prohibitive light burden in optical imaging, whereas electron microscopy is inherently static. Here we solved these challenges by developing an integrated optical/machine-learning technology, LIONESS (live information-optimized nanoscopy enabling saturated segmentation). This leverages optical modifications to stimulated emission depletion microscopy in comprehensively, extracellularly labeled tissue and previous information on sample structure via machine learning to simultaneously achieve isotropic super-resolution, high signal-to-noise ratio and compatibility with living tissue. This allows dense deep-learning-based instance segmentation and 3D reconstruction at a synapse level, incorporating molecular, activity and morphodynamic information. LIONESS opens up avenues for studying the dynamic functional (nano-)architecture of living brain tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.