ArticleNature methods2023
A positively tuned voltage indicator for extended electrical recordings in the brain.
Article in Nature methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 81 citations in OpenAlex.
- Sub-millivolt voltage imaging reveals gap junction-mediated bioelectric contact inhibition.Nature communications · 2026Article
- Crosstalk-free optical indicator-actuator combinations for bidirectional optogenetics.npj biomedical innovations · 2026Review
- Connectomic evidence that ordered activity drives neuromuscular network formation.Nature neuroscience · 2026Article
- A versatile platform for two-photon neuronal population voltage imaging across cortical depths.Nature methods · 2026Article
- Precise calcium-to-spike inference using biophysical generative models.bioRxiv : the preprint server for biology · 2026Article
- A red-emitting, genetically encoded indicator for two-photon voltage recording in vivo.bioRxiv : the preprint server for biology · 2026Article
- Fast analysis and engineering of protein function by microbe-independent deep assembly and screening.Molecular systems biology · 2026Article
- Article
- Improving positively tuned voltage indicators for faster kinetics and higher contrast.bioRxiv : the preprint server for biology · 2026Article
- A versatile, positive-going voltage indicator that enables accessible two-photon recordings in vivo.bioRxiv : the preprint server for biology · 2026Article
- Multimodal optical imaging combining voltage-sensitive dye ElectroFluor630 with genetically encoded calcium, glutamate, or voltage indicators.Neurophotonics · 2026Article
- Multi-scale imaging and recording of in-vivo neural activity.BMB reports · 2026Review
- MiniFAST: a sensitive and fast miniaturized microscope forNeurophotonics · 2026Article
- Bright calcium-modulated bioluminescent indicators for activity imaging and red photon-assisted synaptic transmission.bioRxiv : the preprint server for biology · 2026Article
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
- High-speed neural imaging with multiplexed miniaturized two-photon microscopy.Cell reports methods · 2025Article
- Review
- All-optical voltage interrogation for probing synaptic plasticity in vivo.Nature communications · 2025Article
- In vivo Pirt-Marina voltage sensor imaging detects primary sensory neuron-specific voltage dynamics and neuronal plasticity changes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Voltage imaging reveals hippocampal inhibitory dynamics shaping pyramidal memory-encoding sequences.Nature neuroscience · 2025Article
Corrections and comments
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Authors and funding
33 authors at 8 institutions in 3 countries.
Funding
Abstract
Genetically encoded voltage indicators (GEVIs) enable optical recording of electrical signals in the brain, providing subthreshold sensitivity and temporal resolution not possible with calcium indicators. However, one- and two-photon voltage imaging over prolonged periods with the same GEVI has not yet been demonstrated. Here, we report engineering of ASAP family GEVIs to enhance photostability by inversion of the fluorescence-voltage relationship. Two of the resulting GEVIs, ASAP4b and ASAP4e, respond to 100-mV depolarizations with ≥180% fluorescence increases, compared with the 50% fluorescence decrease of the parental ASAP3. With standard microscopy equipment, ASAP4e enables single-trial detection of spikes in mice over the course of minutes. Unlike GEVIs previously used for one-photon voltage recordings, ASAP4b and ASAP4e also perform well under two-photon illumination. By imaging voltage and calcium simultaneously, we show that ASAP4b and ASAP4e can identify place cells and detect voltage spikes with better temporal resolution than commonly used calcium indicators. Thus, ASAP4b and ASAP4e extend the capabilities of voltage imaging to standard one- and two-photon microscopes while improving the duration of voltage recordings.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.