ArticleMethods in molecular biology (Clifton, N.J.)2023
Whole-Mount In Situ Hybridization in Large Sexual Schmidtea mediterranea.
Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed.
- Drift of positional identity drives reproductive aging in a long-lived regenerative animal.Current biology : CB · 2026Article
- Evolutionary dynamics of whole-body regeneration across planarian flatworms.Nature ecology & evolution · 2023Article
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3 authors.
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Abstract
Whole-mount in situ hybridization (WISH), colorimetric or fluorescent (FISH), allows for the visualization of endogenous RNA. For planarians, robust WISH protocols exist for small-sized animals (>5 mm) of the model species Schmidtea mediterranea and Dugesia japonica. However, the sexual strain of Schmidtea mediterranea studied for germline development and function reaches much larger body sizes in excess of 2 cm. The existing whole-mount WISH protocols are not optimal for such large specimens, owing to insufficient tissue permeabilization. Here, we describe a robust WISH protocol for 12-16 mm long sexually mature Schmidtea mediterranea individuals that could serve as a starting point for adapting WISH to other large planarian species.
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