Evidence map›Paper›PMID 37426764›Full record

ArticleCell reports methods2023

Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay.

Arend W Overeem, Yolanda W Chang, Ioannis Moustakas, Celine M Roelse, Sanne Hillenius, Talia Van Der Helm, Valérie F Van Der Schrier, Manuel A F V Gonçalves, Hailiang Mei, Christian Freund and 1 more

Abstract read
In one paragraph

Article in Cell reports methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Review
  6. Review
  7. Mechanisms of human germ cell development.Nature reviews. Molecular cell biology · 2026
    Review
  8. Proteome of the testicular cell-conditioned medium supports germ cell differentiationJournal of advanced veterinary and animal research · 2025
    Article
  9. Review
  10. Article
  11. Article
  12. Review
  13. Article
  14. Review
  15. Article
  16. Review
  17. Article
  18. Primate amnion development.Development (Cambridge, England) · 2024
    Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Arend W OvereemDepartment of Anatomy and Embryology, Leiden University Medical Center, 2333 ZC Leiden, the Netherlands.
Yolanda W ChangDepartment of Anatomy and Embryology, Leiden University Medical Center, 2333 ZC Leiden, the Netherlands.
Ioannis MoustakasDepartment of Anatomy and Embryology, Leiden University Medical Center, 2333 ZC Leiden, the Netherlands.
Celine M RoelseDepartment of Anatomy and Embryology, Leiden University Medical Center, 2333 ZC Leiden, the Netherlands.
Sanne HilleniusDepartment of Anatomy and Embryology, Leiden University Medical Center, 2333 ZC Leiden, the Netherlands.
Talia Van Der HelmDepartment of Anatomy and Embryology, Leiden University Medical Center, 2333 ZC Leiden, the Netherlands.
Valérie F Van Der SchrierDepartment of Anatomy and Embryology, Leiden University Medical Center, 2333 ZC Leiden, the Netherlands.
Manuel A F V GonçalvesDepartment of Cell and Chemical Biology, Leiden University Medical Center, 2333 ZC Leiden, the Netherlands.
Hailiang MeiSequencing Analysis Support Core, Leiden University Medical Center, 2333 ZC Leiden, the Netherlands.
Christian FreundDepartment of Anatomy and Embryology, Leiden University Medical Center, 2333 ZC Leiden, the Netherlands.
Susana M Chuva de Sousa LopesDepartment of Anatomy and Embryology, Leiden University Medical Center, 2333 ZC Leiden, the Netherlands.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Current methods to generate human primordial germ cell-like cells (hPGCLCs) from human pluripotent stem cells (hPSCs) can be inefficient, and it is challenging to generate sufficient hPGCLCs to optimize

Indexed as

Induced Pluripotent Stem CellsPluripotent Stem CellsCell DifferentiationFemaleGerm CellsHumansOvaryamnionbasement membrane extractBMPdifferentiationhumanin vitro gametogenesispluripotent stem cellsprimordial germ cells

Identifiers

PMID37426764
PMCPMC10326346

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.