Evidence map›Paper›PMID 37425882›Full record

ArticlebioRxiv : the preprint server for biology2023

Rapid assessment of changes in phage bioactivity using dynamic light scattering.

Tejas Dharmaraj, Michael J Kratochvil, Julie D Pourtois, Qingquan Chen, Maryam Hajfathalian, Aviv Hargil, Yung-Hao Lin, Zoe Evans, Agnès Oromí-Bosch, Joel D Berry and 10 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

20 authors.

Tejas DharmarajDivision of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
Michael J KratochvilDepartment of Materials Science and Engineering, Stanford University, Stanford, CA 94305.
Julie D PourtoisHopkins Marine Station, Department of Biology, Stanford University, Pacific Grove, CA 93950, USA.
Qingquan ChenDivision of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
Maryam HajfathalianDivision of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
Aviv HargilDivision of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
Yung-Hao LinDepartment of Chemical Engineering, Stanford University, Stanford, CA 94305, USA.
Zoe EvansDivision of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
Agnès Oromí-BoschFelix Biotechnology, South San Francisco, CA, 94080.
Joel D BerryFelix Biotechnology, South San Francisco, CA, 94080.
Robert McBrideFelix Biotechnology, South San Francisco, CA, 94080.
Naomi L HaddockDivision of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
Derek R HolmanDivision of Gastroenterology and Hepatology, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
Jonas D van BelleghemDivision of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
Tony H ChangDivision of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
Jeremy J BarrSchool of Biological Sciences, Monash University, Clayton, 3800, VIC, Australia.
Rob LavigneDepartment of Biosystems, KU Leuven, Leuven, 3001, Belgium.
Sarah C HeilshornDepartment of Materials Science and Engineering, Stanford University, Stanford, CA 94305.
Francis G BlankenbergDivision of Pediatric Radiology and Nuclear Medicine, Department of Radiology, Lucile Packard Children's Hospital, Stanford, CA 94305, USA.
Paul L BollykyDivision of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.

Funding

MEDICAL SCIENTIST TRAINING PROGRAMT32GM007365 · NIGMS · STANFORD UNIVERSITY · PI CHUA, KATRIN F · 1985 to 2021
$33.8M
The role of macrophages in chronic suppurative otitis media associated sensory hearing lossR01DC019965 · NIDCD · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Peter Luke Santa Maria · 2022 to 2026
$3.6M
Pathogenesis of Pf Bacteriophages in Pseudomonas Cystic Fibrosis lung InfectionsR01HL148184 · NHLBI · STANFORD UNIVERSITY · PI MILLA, CARLOS · 2019 to 2022
$1.7M
NHLBI NIH HHS R01 HL148184NIDCD NIH HHS R01 DC019965NIGMS NIH HHS T32 GM007365
6 · The paper itself

Abstract

Extensive efforts are underway to develop bacteriophages as therapies against antibiotic-resistant bacteria. However, these efforts are confounded by the instability of phage preparations and a lack of suitable tools to assess active phage concentrations over time. Here, we use Dynamic Light Scattering (DLS) to measure changes in phage physical state in response to environmental factors and time, finding that phages tend to decay and form aggregates and that the degree of aggregation can be used to predict phage bioactivity. We then use DLS to optimize phage storage conditions for phages from human clinical trials, predict bioactivity in 50-year-old archival stocks, and evaluate phage samples for use in a phage therapy/wound infection model. We also provide a web-application (Phage-ELF) to facilitate DLS studies of phages. We conclude that DLS provides a rapid, convenient, and non-destructive tool for quality control of phage preparations in academic and commercial settings.

Indexed as

aggregationbacteriophage decaybacteriophage therapyBiophysics and Computational Biology (Biological, Health, and Medical Sciences)dynamic light scattering

Identifiers

PMID37425882
PMCPMC10327207

What OpenQuestion holds

Textmetadata
LicenceCC BY-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.