Evidence map›Paper›PMID 37425880›Full record

ArticlebioRxiv : the preprint server for biology2023

Interaction between host G3BP and viral nucleocapsid protein regulates SARS-CoV-2 replication.

Zemin Yang, Bryan A Johnson, Victoria A Meliopoulos, Xiaohui Ju, Peipei Zhang, Michael P Hughes, Jinjun Wu, Kaitlin P Koreski, Ti-Cheng Chang, Gang Wu and 17 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 8 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

27 authors at 3 institutions in 2 countries.

Zemin YangDepartment of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Bryan A JohnsonDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA.
Victoria A MeliopoulosDepartment of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN, USA.
Xiaohui JuSchool of Medicine, Tsinghua University, Beijing, China.
Peipei ZhangDepartment of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Michael P HughesDepartment of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Jinjun WuDepartment of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Kaitlin P KoreskiDepartment of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Ti-Cheng ChangCenter for Applied Bioinformatics, St. Jude Children's Research Hospital, Memphis, TN, USA.
Gang WuCenter for Applied Bioinformatics, St. Jude Children's Research Hospital, Memphis, TN, USA.
Jeff HixonFaze Medicines, Cambridge, MA, USA.
Jay DuffnerFaze Medicines, Cambridge, MA, USA.
Kathy WongFaze Medicines, Cambridge, MA, USA.
Rene LemieuxFaze Medicines, Cambridge, MA, USA.
Kumari G LokugamageDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA.
Rojelio E AlvardoDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA.
Patricia A Crocquet-ValdesDepartment of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
David H WalkerDepartment of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
Kenneth S PlanteWorld Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, TX, USA.
Jessica A PlanteWorld Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, TX, USA.
Scott C WeaverWorld Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, TX, USA.
Hong Joo KimDepartment of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Rachel MeyersFaze Medicines, Cambridge, MA, USA.
Stacey Schultz-CherryDepartment of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN, USA.
Qiang DingSchool of Medicine, Tsinghua University, Beijing, China.
Vineet D MenacheryDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA.
J Paul TaylorDepartment of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN, USA.
St. Jude Children's Research Hospital · USThe University of Texas Medical Branch at Galveston · USTsinghua University · CN

Funding

Dynamic RNA-protein assemblies and neurological diseaseR35NS097974 · NINDS · ST. JUDE CHILDREN'S RESEARCH HOSPITAL · PI TAYLOR, JOSEPH PAUL · 2017 to 2024
$7.2M
NINDS NIH HHS R35 NS097974
6 · The paper itself

Abstract

G3BP1/2 are paralogous proteins that promote stress granule formation in response to cellular stresses, including viral infection. G3BP1/2 are prominent interactors of the nucleocapsid (N) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, the functional consequences of the G3BP1-N interaction in the context of viral infection remain unclear. Here we used structural and biochemical analyses to define the residues required for G3BP1-N interaction, followed by structure-guided mutagenesis of G3BP1 and N to selectively and reciprocally disrupt their interaction. We found that mutation of F17 within the N protein led to selective loss of interaction with G3BP1 and consequent failure of the N protein to disrupt stress granule assembly. Introduction of SARS-CoV-2 bearing an F17A mutation resulted in a significant decrease in viral replication and pathogenesis in vivo, indicating that the G3BP1-N interaction promotes infection by suppressing the ability of G3BP1 to form stress granules.

Identifiers

PMID37425880
PMCPMC10327126
OpenAlexW4382652045

What OpenQuestion holds

Textmetadata
LicenceCC BY-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.