Evidence map›Paper›PMID 37425699›Full record

ArticlebioRxiv : the preprint server for biology2025

Expansion-assisted selective plane illumination microscopy for nanoscale imaging of centimeter-scale tissues.

Adam Glaser, Jayaram Chandrashekar, Sonya Vasquez, Cameron Arshadi, Rajvi Javeri, Naveen Ouellette, Xiaoyun Jiang, Judith Baka, Gabor Kovacs, Micah Woodard and 24 more

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Current Status and Future Strategies for Advancing Functional Circuit MappingThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2023
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

34 authors.

Jayaram ChandrashekarORCID 0000-0001-6412-0114
Rajvi Javeri
Naveen Ouellette
Micah Woodard
Sharmishtaa SeshamaniORCID 0000-0001-7289-3184
Andrew Recknagel
Anna Grim
Marcus Hooper
Alan Liddell
John Rohde
Ayana Hellevik
Kevin Takasaki
Lindsey Erion BarnerORCID 0000-0002-9156-0024
Molly Logsdon
Chris ChronopoulosORCID 0009-0000-1989-1876
Bosiljka Tasic

Funding

Scalable technologies for brain-wide connectomics of transcriptomic cell types: focus on brainstemRF1MH128841 · NIMH · ALLEN INSTITUTE · PI CHANDRASHEKAR, JAYARAM, SVOBODA, KAREL · 2022 to 2022
$4.7M
Multimodal analysis of primate infragranular pyramidal neurons and their modulationR01NS123959 · NINDS · ALLEN INSTITUTE · PI DEMBROW, NIKOLAI C, KALMBACH, BRIAN E. · 2021 to 2025
$2.8M
Light-sheet micro-aspiration microscopy for the isolation and analysis of rare tumor cells from intact clinical specimensR00CA240681 · NCI · ALLEN INSTITUTE · PI GLASER, ADAM K · 2021 to 2023
$745k
NCI NIH HHS R00 CA240681NIMH NIH HHS RF1 MH128841NINDS NIH HHS R01 NS123959
6 · The paper itself

Abstract

Recent advances in tissue processing, labeling, and fluorescence microscopy are providing unprecedented views of the structure of cells and tissues at sub-diffraction resolutions and near single molecule sensitivity, driving discoveries in diverse fields of biology, including neuroscience. Biological tissue is organized over scales of nanometers to centimeters. Harnessing molecular imaging across intact, three-dimensional samples on this scale requires new types of microscopes with larger fields of view and working distance, as well as higher throughput. We present a new expansion-assisted selective plane illumination microscope (ExA-SPIM) with aberration-free 1.5 µm×1.5 µm×3 µm optical resolution over a large field of view (10.6×8.0 mm

Identifiers

PMID37425699
PMCPMC10327101

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.