ArticlebioRxiv : the preprint server for biology2024
Abcg2a is the functional homolog of human ABCG2 expressed at the zebrafish blood-brain barrier.
Joanna R Thomas, William J E Frye, Robert W Robey, Andrew C Warner, Donna Butcher, Jennifer L Matta, Tamara C Morgan, Elijah F Edmondson, Paula B Salazar, Suresh V Ambudkar and 1 more
Open access · greenAbstract readPreprint
In one paragraphArticle in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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0 citing papers in PubMed, 2 citations in OpenAlex.
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4 · The recordCorrections and comments
5 · Who and what moneyAuthors and funding
11 authors at 2 institutions in 1 country.
Joanna R ThomasLaboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.ORCID 0000-0002-0508-7358 William J E FryeLaboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Robert W RobeyLaboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Andrew C WarnerMolecular Histopathology Laboratory, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
Donna ButcherMolecular Histopathology Laboratory, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
Jennifer L MattaMolecular Histopathology Laboratory, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
Tamara C MorganMolecular Histopathology Laboratory, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
Elijah F EdmondsonMolecular Histopathology Laboratory, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.ORCID 0000-0002-6106-3705 Paula B SalazarLaboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.ORCID 0000-0001-7280-6968 Suresh V AmbudkarLaboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.ORCID 0000-0002-2639-4955 Michael M GottesmanLaboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.ORCID 0000-0001-8908-2097 National Institutes of Health · USFrederick National Laboratory for Cancer Research · US
Funding
WORK ORDER 126643 B539 EXPAND IC SUITE75N91019D00024 · NIAID · LEIDOS BIOMEDICAL RESEARCH, INC. · PI PENDLETON, RICHARD · 2019 to 2025
$3932.6MBiochemical Analysis of Multidrug Resistance-linked Transport ProteinsZIABC010030 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI AMBUDKAR, SURESH · 2009 to 2025
$21.6MZebrafish model of blood-brain barrier to improve drug delivery to the brainZIABC012052 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI GOTTESMAN, MICHAEL · 2021 to 2025
$3.5MNCI NIH HHS 75N91019D00024
6 · The paper itselfAbstract
Background: A principal protective component of the mammalian blood-brain barrier (BBB) is the high expression of the multidrug efflux transporters P-glycoprotein (P-gp, encoded by Methods: To determine substrates of the transporters, we stably expressed each in HEK-293 cells and performed cytotoxicity and fluorescent efflux assays with known ABCG2 substrates. To assess the expression of transporter homologs, we used a combination of RNAscope Results: We found Abcg2a had the greatest substrate overlap with ABCG2, and Abcg2d appeared to be the least functionally similar. We identified Conclusions: These results demonstrate the conserved function of zebrafish Abcg2a and suggest that zebrafish may be an appropriate model organism for the studying the role of ABCG2 at the BBB.
Indexed as
ABCG2Blood-brain barrierdrug resistanceZebrafish
Identifiers
PMID37425689
PMCPMC10327217
OpenAlexW4377098550
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