Evidence map›Paper›PMID 37424948›Full record

ArticleWorld journal of stem cells2023

Culture and identification of neonatal rat brain-derived neural stem cells.

Qing-Zhong Zhou, Xiao-Lan Feng, Xu-Feng Jia, Nurul Huda Binti Mohd Nor, Mohd Hezery Bin Harun, Da-Xiong Feng, Wan Aliaa Wan Sulaiman

Open access · hybridAbstract read
In one paragraph

Article in World journal of stem cells, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Qing-Zhong ZhouDepartment of Orthopedics, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China.
Xiao-Lan FengDepartment of Radiology, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China.
Xu-Feng JiaDepartment of Orthopedics, The Peoples' Hospital of Jianyang City, Jianyang 641400, Sichuan Province, China.
Nurul Huda Binti Mohd NorDepartment of Human Anatomi, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang Selangor, 43400, Malaysia.
Mohd Hezery Bin HarunDepartment of Orthopedics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
Da-Xiong FengDepartment of Orthopedics, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China.
Wan Aliaa Wan SulaimanDepartment of Neurology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. wanaliaa@upm.edu.my.
Affiliated Hospital of Southwest Medical University · CNUniversiti Putra Malaysia · MY

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundTiming of passaging, passage number, passaging approaches and methods for cell identification are critical factors influencing the quality of neural stem cells (NSCs) culture. How to effectively culture and identify NSCs is a continuous interest in NSCs study while these factors are comprehensively considered.

aimTo establish a simplified and efficient method for culture and identification of neonatal rat brain-derived NSCs.

methodsFirst, curved tip operating scissors were used to dissect brain tissues from new born rats (2 to 3 d) and the brain tissues were cut into approximately 1 mm

resultsBrain derived cells from newborn rats (2 to 3 d) proliferate and aggregate into spherical-shaped clusters with sustained continuous and stable passaging. When BrdU was incorporated into the 5

conclusionThis is a simplified and efficient method for neonatal rat brain-derived neural stem cell culture and identification.

Indexed as

Brain-derived neural stem cellsCultureIdentificationNeonatal rats

Identifiers

PMID37424948
PMCPMC10324507
OpenAlexW4382066009

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.