Evidence map›Paper›PMID 37423923›Full record

ArticleScientific reports2023

Discovery and optimized extraction of the anti-osteoclastic agent epicatechin-7-O-β-D-apiofuranoside from Ulmus macrocarpa Hance bark.

Chanhyeok Jeong, Yeon-Jin Cho, Yongjin Lee, Weihong Wang, Kyu-Hyung Park, Eun Roh, Chang Hyung Lee, Young-Jin Son, Jung Han Yoon Park, Heonjoong Kang and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Chanhyeok Jeong *Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Korea.
Yeon-Jin Cho *Bio-MAX Institute, Seoul National University, Seoul, 08826, Korea.
Yongjin Lee *Department of Pharmacy, Sunchon National University, 315 Maegok-dong, Suncheon, Jeollanam-do, 57922, Korea.
Weihong WangLaboratory of Marine Drugs, School of Earth and Environmental Sciences, Seoul National University, NS-80, Seoul, 08826, Korea.
Kyu-Hyung ParkLaboratory of Marine Drugs, School of Earth and Environmental Sciences, Seoul National University, NS-80, Seoul, 08826, Korea.
Eun RohLaboratory of Marine Drugs, School of Earth and Environmental Sciences, Seoul National University, NS-80, Seoul, 08826, Korea.
Chang Hyung LeeBio-MAX Institute, Seoul National University, Seoul, 08826, Korea.
Young-Jin SonDepartment of Pharmacy, Sunchon National University, 315 Maegok-dong, Suncheon, Jeollanam-do, 57922, Korea.
Jung Han Yoon ParkBio-MAX Institute, Seoul National University, Seoul, 08826, Korea.
Heonjoong KangBio-MAX Institute, Seoul National University, Seoul, 08826, Korea.
Ki Won LeeDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Korea. kiwon@snu.ac.kr.
Seoul National University · KRSunchon National University · KRSeoul National University of Science and Technology · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ulmus macrocarpa Hance bark (UmHb) has been used as a traditional herbal medicine in East Asia for bone concern diseases for a long time. To find a suitable solvent, we, in this study, compared the efficacy of UmHb water extract and ethanol extract which can inhibit osteoclast differentiation. Compared with two ethanol extracts (70% and 100% respectively), hydrothermal extracts of UmHb more effectively inhibited receptor activators of nuclear factor κB ligand-induced osteoclast differentiation in murine bone marrow-derived macrophages. We identified for the first time that (2R,3R)-epicatechin-7-O-β-D-apiofuranoside (E7A) is a specific active compound in UmHb hydrothermal extracts through using LC/MS, HPLC, and NMR techniques. In addition, we confirmed through TRAP assay, pit assay, and PCR assay that E7A is a key compound in inhibiting osteoclast differentiation. The optimized condition to obtain E7A-rich UmHb extract was 100 mL/g, 90 °C, pH 5, and 97 min. At this condition, the content of E7A was 26.05 ± 0.96 mg/g extract. Based on TRAP assay, pit assay, PCR, and western blot, the optimized extract of E7A-rich UmHb demonstrated a greater inhibition of osteoclast differentiation compared to unoptimized. These results suggest that E7A would be a good candidate for the prevention and treatment of osteoporosis-related diseases.

Indexed as

CatechinUlmusAnimalsCell DifferentiationEthanolMiceOsteoclastsPlant BarkPlant ExtractsRANK LigandCatechinEthanolPlant ExtractsRANK Ligand

Identifiers

PMID37423923
PMCPMC10330169
OpenAlexW4383682241

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.