ArticleMed (New York, N.Y.)2023
SARS-CoV-2 niches in human placenta revealed by spatial transcriptomics.
Article in Med (New York, N.Y.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 29 citations in OpenAlex.
- Divide-and-conquer analysis reveals hidden immune cell influencers across the placenta in preeclampsia.BioData mining · 2026Article
- Single-Cell and Spatial Omics: Methods and Applications.MedComm · 2026Review
- Review
- COVID-19-related inflammation of the placenta impedes fetal development in pregnant hamsters.Nature communications · 2026Article
- Spatial microenvironments tune immune response dynamics in the Drosophila larval fat body.PLoS genetics · 2026Article
- Innate immune responses to pathogens at the maternal-fetal interface.Nature reviews. Immunology · 2025Review
- A survey of SARS-CoV-2 tropism.Infectious diseases & immunity · 2025Review
- Spatial microenvironments tune immune response dynamics in thebioRxiv : the preprint server for biology · 2025Article
- Spatial Transcriptomics to Study Virus-Host Interactions.Annual review of virology · 2025Review
- A Human Immuno-Lung Organoid Model to Study Macrophage-Mediated Lung Cell Senescence Upon SARS-CoV-2 Infection.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Spatial transcriptomics: a bibliometric analysis with large language model on English literatures.Briefings in bioinformatics · 2025Article
- The respiratory tract virome: unravelling the role of viral dark matter in respiratory health and disease.European respiratory review : an official journal of the European Respiratory Society · 2025Review
- Single-nucleus transcriptional profiling of the placenta reveals the syncytiotrophoblast stress response to COVID-19.American journal of obstetrics and gynecology · 2025Article
- The placental battlefield: viral strategies and immune countermeasures.Frontiers in immunology · 2025Review
- Human vascularized macrophage-islet organoids to model immune-mediated pancreatic β cell pyroptosis upon viral infection.Cell stem cell · 2024Article
- Dual spatial host-bacterial gene expression in Mycobacterium abscessus respiratory infections.Communications biology · 2024Article
- Transplacental Transmission of SARS-CoV-2: A Narrative Review.Medicina (Kaunas, Lithuania) · 2024Review
- Fetal MAVS and type I IFN signaling pathways control ZIKV infection in the placenta and maternal decidua.The Journal of experimental medicine · 2024Article
- Single-cell transcriptomic analysis of immune cell dynamics in the healthy human endometrium.Biochemistry and biophysics reports · 2024Article
- Human Vascularized Macrophage-Islet Organoids to Model Immune-Mediated Pancreatic β cell Pyroptosis upon Viral Infection.bioRxiv : the preprint server for biology · 2024Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundFunctional placental niches are presumed to spatially separate maternal-fetal antigens and restrict the vertical transmission of pathogens. We hypothesized a high-resolution map of placental transcription could provide direct evidence for niche microenvironments with unique functions and transcription profiles.
methodsWe utilized Visium Spatial Transcriptomics paired with H&E staining to generate 17,927 spatial transcriptomes. By integrating these spatial transcriptomes with 273,944 placental single-cell and single-nuclei transcriptomes, we generated an atlas composed of at least 22 subpopulations in the maternal decidua, fetal chorionic villi, and chorioamniotic membranes.
findingsComparisons of placentae from uninfected healthy controls (n = 4) with COVID-19 asymptomatic (n = 4) and symptomatic (n = 5) infected participants demonstrated that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection in syncytiotrophoblasts occurred in both the presence and the absence of maternal clinical disease. With spatial transcriptomics, we found that the limit of detection for SARS-CoV-2 was 1/7,000 cells, and placental niches without detectable viral transcripts were unperturbed. In contrast, niches with high SARS-CoV-2 transcript levels were associated with significant upregulation in pro-inflammatory cytokines and interferon-stimulated genes, altered metallopeptidase signaling (TIMP1), with coordinated shifts in macrophage polarization, histiocytic intervillositis, and perivillous fibrin deposition. Fetal sex differences in gene expression responses to SARS-CoV-2 were limited, with confirmed mapping limited to the maternal decidua in males.
conclusionsHigh-resolution placental transcriptomics with spatial resolution revealed dynamic responses to SARS-CoV-2 in coordinate microenvironments in the absence and presence of clinically evident disease.
fundingThis work was supported by the NIH (R01HD091731 and T32-HD098069), NSF (2208903), the Burroughs Welcome Fund and the March of Dimes Preterm Birth Research Initiatives, and a Career Development Award from the American Society of Gene and Cell Therapy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.