ArticleScience advances2023
Single-cell and spatial transcriptomics identify a macrophage population associated with skeletal muscle fibrosis.
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Article in Science advances, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
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99citing papers in PubMed
18.8field-weighted citation impact, top 1% of its field
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Who cites it
99 citing papers in PubMed, 112 citations in OpenAlex.
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- BACH1 orchestrates macrophage state transitions to coordinate regenerative inflammation.Journal of immunology (Baltimore, Md. : 1950) · 2026Article
- Transcriptomic profiling of the ovarian immune landscape reveals distinct macrophage subsets and activation of the NLRP3 inflammasome likely contributing to accelerated follicular atresia in classic galactosemia.Cell communication and signaling : CCS · 2026Article
- Decoding cellular communication networks and signaling pathways in bone, skeletal muscle, and bone-muscle crosstalk through spatial transcriptomics in a young male mouse.Bone research · 2026Article
39 more citing papers are in PubMed but not listed here.
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Authors and funding
35 authors at 9 institutions in 1 country.
Gerald CoulisDepartment of Physiology and Biophysics, University of California Irvine, Irvine, CA, USA.ORCID 0000-0001-5991-9706
Diego JaimeDepartment of Physiology and Biophysics, University of California Irvine, Irvine, CA, USA.ORCID 0000-0002-8903-9168
Christian Guerrero-JuarezCarle Illinois College of Medicine, University of Illinois at Urbana-Champaign, Champaign, IL, USA.ORCID 0000-0002-6245-6412
Jenna M KastenschmidtDepartment of Physiology and Biophysics, University of California Irvine, Irvine, CA, USA.ORCID 0000-0002-0067-5142
Philip K FarahatDepartment of Physiology and Biophysics, University of California Irvine, Irvine, CA, USA.ORCID 0000-0001-7481-0581
Quy NguyenDepartment of Biological Chemistry, University of California Irvine, Irvine, CA USA.
Nicholas PervolarakisDepartment of Biological Chemistry, University of California Irvine, Irvine, CA USA.ORCID 0000-0001-6210-4022
Katherine McLindenDepartment of Molecular Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA, USA.
Lauren ThurlowDepartment of Molecular Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA, USA.ORCID 0000-0002-2832-0300
Saba MovahediDepartment of Physiology and Biophysics, University of California Irvine, Irvine, CA, USA.
Brandon S HughesDepartment of Physiology and Biophysics, University of California Irvine, Irvine, CA, USA.ORCID 0000-0003-0424-2392
Jorge DuarteDepartment of Physiology and Biophysics, University of California Irvine, Irvine, CA, USA.
Andrew SornDepartment of Physiology and Biophysics, University of California Irvine, Irvine, CA, USA.ORCID 0000-0001-8660-1533
Elizabeth MontoyaDepartment of Physiology and Biophysics, University of California Irvine, Irvine, CA, USA.ORCID 0000-0002-7306-5724
Izza MozaffarDepartment of Physiology and Biophysics, University of California Irvine, Irvine, CA, USA.
Morgan DraganDepartment of Biological Chemistry, University of California Irvine, Irvine, CA USA.ORCID 0000-0002-6401-6816
Shivashankar OthyDepartment of Physiology and Biophysics, University of California Irvine, Irvine, CA, USA.ORCID 0000-0001-6832-5547
Trupti JoshiDepartment of Health Management and Informatics, University of Missouri, Columbia, MO, USA.ORCID 0000-0001-8944-4924
Chetan P HansDepartment of Cardiovascular Medicine, University of Missouri, Columbia, MO USA.ORCID 0000-0003-1830-9084
Virginia KimonisDepartment of Pediatrics, University of California Irvine, Irvine, CA, USA.ORCID 0000-0003-1567-4449
Adam L MacLeanDepartment of Quantitative and Computational Biology, University of Southern California, Los Angeles, CA, USA.ORCID 0000-0003-0689-7907
Qing NieDepartment of Mathematics, Department of Developmental and Cell Biology, University of California Irvine, Irvine, CA, USA.ORCID 0000-0002-8804-3368
Lindsay M WallaceCenter for Gene Therapy, The Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, OH, USA.ORCID 0000-0001-6780-7464
Scott Q HarperCenter for Gene Therapy, The Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, OH, USA.
Tahseen MozaffarDepartment of Neurology, University of California Irvine, Irvine, CA, USA.ORCID 0000-0002-1230-0188
Marshall W HogarthChildren's National Hospital, Research Center for Genetic Medicine, Washington, DC, USA.ORCID 0000-0003-1273-3773
Surajit BhattacharyaChildren's National Hospital, Research Center for Genetic Medicine, Washington, DC, USA.ORCID 0000-0001-5589-4652
Jyoti K JaiswalChildren's National Hospital, Research Center for Genetic Medicine, Washington, DC, USA.ORCID 0000-0002-5992-5185
David R GolannRegeneron Pharmaceuticals Inc., Tarrytown, NY, USA.
Qi SuRegeneron Pharmaceuticals Inc., Tarrytown, NY, USA.
Kai KessenbrockDepartment of Biological Chemistry, University of California Irvine, Irvine, CA USA.ORCID 0000-0003-0410-6104
Michael StecRegeneron Pharmaceuticals Inc., Tarrytown, NY, USA.ORCID 0000-0002-0296-4984
Melissa J SpencerDepartment of Neurology, University of California Los Angeles, Los Angeles, CA, USA.ORCID 0000-0001-7104-544X
Jesse R ZamudioDepartment of Molecular Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA, USA.ORCID 0000-0001-8714-1049
S Armando VillaltaDepartment of Physiology and Biophysics, University of California Irvine, Irvine, CA, USA.ORCID 0000-0002-5212-2259
University of California, Irvine · USUniversity of California, Los Angeles · USChildren's National · USRegeneron (United States) · USNationwide Children's Hospital · USUniversity of Illinois Urbana-Champaign · USUniversity of Missouri · USUniversity of Missouri Health System · USUniversity of Southern California · US
Funding
Univ.of Calif., Irvine Cancer Center Support GrantP30CA062203 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI Melanie Funes · 1994 to 2026
$57.9MUniversity of California Health Participation in the National COVID Cohort Collaborative (N3C)UL1TR001414 · NCATS · UNIVERSITY OF CALIFORNIA-IRVINE · PI COOPER, DAN M, VILAIN, ERIC J. · 2015 to 2023
$35.1MTraining and education CoreU54AR052646 · NIAMS · UNIVERSITY OF PENNSYLVANIA · PI MCNALLY, ELIZABETH M, SWEENEY, H LEE · 2005 to 2020
$24.1MUnderstanding and Improving Therapies for the Muscular Dystrophies through Noninvasive BiomarkersP50AR052646 · NIAMS · UNIVERSITY OF FLORIDA · PI SWEENEY, H LEE · 2021 to 2024
$6.2MInstitutional Career Development CoreKL2TR001416 · NCATS · UNIVERSITY OF CALIFORNIA-IRVINE · PI CAIOZZO, VINCENT JAMES, DEMETRIOU, MICHAEL · 2015 to 2023
$5.6MUCI P30 Skin Center Systems Biology CoreP30AR075047 · NIAMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI LANDER, ARTHUR D · 2019 to 2025
$5.1MInfluence of NT5c1A antibodies on disease progression, clinical phenotype and blood and muscle biomarkers in sporadic Inclusion Body Myositis - A prospective evaluationR01AR078340 · NIAMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI MOZAFFAR, TAHSEEN · 2021 to 2025
$4.2MUnderstanding the mechanism and role of cell membrane repair in Miyoshi MyopathyR01AR055686 · NIAMS · ROCKEFELLER UNIVERSITY · PI JAISWAL, JYOTI K · 2008 to 2021
$3.6MTissue-Specific Mechanisms of Regulatory T Cells in the CNS during Autoimmune EncephalomyelitisR01AI168063 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI Shivashankar Othy · 2022 to 2026
$2.5MNovel macrophage and regulatory T cell interactions that promote the pathogenesis of Duchenne muscular dystrophyR01NS120060 · NINDS · UNIVERSITY OF CALIFORNIA-IRVINE · PI VILLALTA, SERGIO ARMANDO · 2020 to 2024
$2.5MComputational methods to predict gene regulatory network dynamics and cell state transitionsR35GM143019 · NIGMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI MACLEAN, ADAM L · 2021 to 2025
$2.1MImmunology Research Training ProgramT32AI060573 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI PEARLMAN, ERIC · 2005 to 2020
$1.9MNCATS NIH HHS KL2 TR001416NCATS NIH HHS UL1 TR001414NCI NIH HHS P30 CA062203NIAID NIH HHS R01 AI168063NIAID NIH HHS T32 AI060573NIAMS NIH HHS P30 AR075047NIAMS NIH HHS P50 AR052646NIAMS NIH HHS R01 AR055686NIAMS NIH HHS R01 AR078340NIAMS NIH HHS U54 AR052646NIGMS NIH HHS R01 GM143536NIGMS NIH HHS R35 GM143019NINDS NIH HHS R01 NS120060
Abstract
Macrophages are essential for skeletal muscle homeostasis, but how their dysregulation contributes to the development of fibrosis in muscle disease remains unclear. Here, we used single-cell transcriptomics to determine the molecular attributes of dystrophic and healthy muscle macrophages. We identified six clusters and unexpectedly found that none corresponded to traditional definitions of M1 or M2 macrophages. Rather, the predominant macrophage signature in dystrophic muscle was characterized by high expression of fibrotic factors, galectin-3 (gal-3) and osteopontin (
Indexed as
MacrophagesTranscriptomeAnimalsFibrosisGalectin 3HumansMiceMice, Inbred C57BLMuscle, SkeletalGalectin 3
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