Evidence map›Paper›PMID 37418145›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Manual Dissociation of Mammalian Preimplantation Embryos for Single-Cell Genomics.

Katherine Vandal, Savana Biondic, Jesica Canizo, Sophie Petropoulos

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Katherine VandalCentre de Recherche du Centre Hospitalier de l'Université de Montréal, Axe Immunopathologie, Montréal, ON, Canada.
Savana BiondicCentre de Recherche du Centre Hospitalier de l'Université de Montréal, Axe Immunopathologie, Montréal, ON, Canada.
Jesica CanizoCentre de Recherche du Centre Hospitalier de l'Université de Montréal, Axe Immunopathologie, Montréal, ON, Canada.
Sophie PetropoulosCentre de Recherche du Centre Hospitalier de l'Université de Montréal, Axe Immunopathologie, Montréal, ON, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single-cell genomics allow the characterization and quantification of molecular heterogeneity from a wide variety of tissues. Here, we describe the manual dissociation and collection of single cells, a method adapted for the characterization of precious small tissues like preimplantation embryos. We also describe the acquisition of mouse embryos by flushing of the oviducts. The cells can then be used in multiple sequencing protocols, for example, Smart-seq2, Smart-seq3, smallseq, and scBSseq.

Indexed as

BlastocystEmbryo, MammalianAnimalsGenomicsMammalsMiceEmbryo collectionHumanMousePreimplantation embryoSequencingSingle-cell

Identifiers

PMID37418145

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.