ArticleApplied microbiology and biotechnology2023
Lactate formation from fructose or C1 compounds in the acetogen Acetobacterium woodii by metabolic engineering.
Article in Applied microbiology and biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 9 citations in OpenAlex.
- Formate as electron carrier in the gut acetogenGut microbes · 2026Article
- Formate- and Sodium Ion-Dependent Wood-Ljungdahl Pathway Involved in Organo-, Litho-, and Methylotrophic Growth of Tepidanaerobacter acetatoxydans.Environmental science & technology · 2026Article
- Improving autotrophic D-lactate production by heterologous expression of a pyruvate formate lyase in an LDHD-expressing Acetobacterium woodii strain.Applied microbiology and biotechnology · 2026Article
- Careful CO Addition Enhances Autotrophic d-Lactate Formation With EngineeredEngineering in life sciences · 2026Article
- Purification and characterization of a thermophilic NADFEBS open bio · 2025Article
- Methanothermobacter thermautotrophicus and Alternative Methanogens: Archaea-Based Production.Advances in biochemical engineering/biotechnology · 2025Review
- Harnessing acetogenic bacteria for one-carbon valorization toward sustainable chemical production.RSC chemical biology · 2024Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Anaerobic, acetogenic bacteria are promising biocatalysts for a sustainable bioeconomy since they capture and convert carbon dioxide to acetic acid. Hydrogen is an intermediate in acetate formation from organic as well as C1 substrates. Here, we analyzed mutants of the model acetogen Acetobacterium woodii in which either one of the two hydrogenases or both together were genetically deleted. In resting cells of the double mutant, hydrogen formation from fructose was completely abolished and carbon was redirected largely to lactate. The lactate/fructose and lactate/acetate ratios were 1.24 and 2.76, respectively. We then tested for lactate formation from methyl groups (derived from glycine betaine) and carbon monoxide. Indeed, also under these conditions lactate and acetate were formed in equimolar amounts with a lactate/acetate ratio of 1.13. When the electron-bifurcating lactate dehydrogenase/ETF complex was genetically deleted, lactate formation was completely abolished. These experiments demonstrate the capability of A. woodii to produce lactate from fructose but also from promising C1 substrates, methyl groups and carbon monoxide. This adds an important milestone towards generation of a value chain leading from CO
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