ArticleFrontiers in cellular neuroscience2023
A simple method for poly-D-lysine coating to enhance adhesion and maturation of primary cortical neuron cultures
Article in Frontiers in cellular neuroscience, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed, 15 citations in OpenAlex.
- Comparative analysis of surface coatings for human neural co-cultures identifies fibronectin as a robust MEA substrate.Scientific reports · 2026Article
- Evaluation of a human 3D multi-spheroid model derived from SH-SY5Y cells for cytotoxicity testing.Scientific reports · 2026Article
- Emerging Approaches to Mitigate Neural Cell Degeneration with Nanoparticles-Enhanced Polyelectrolyte Systems.Membranes · 2025Review
- Functionalized Nanodiamonds for Targeted Neuronal Electromagnetic Signal Detection.ACS applied materials & interfaces · 2024Article
- Gradient Functionalization of Poly(lactic acid)-Based Materials with Polylysine for Spatially Controlled Cell Adhesion.Polymers · 2024Article
- Mimicking the Physicochemical Properties of the Cornea: A Low-Cost Approximation Using Highly Available Biopolymers.Polymers · 2024Article
- Human sensory-like neuron cultivation-An optimized protocol.Frontiers in neuroscience · 2024Article
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Authors and funding
12 authors at 3 institutions in 1 country.
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Abstract
Introduction: Glass coverslips are used as a substrate since Harrison's initial nerve cell culture experiments in 1910. In 1974, the first study of brain cells seeded onto polylysine (PL) coated substrate was published. Usually, neurons adhere quickly to PL coating. However, maintaining cortical neurons in culture on PL coating for a prolonged time is challenging. Methods: A collaborative study between chemical engineers and neurobiologists was conducted to find a simple method to enhance neuronal maturation on poly-D-lysine (PDL). In this work, a simple protocol to coat PDL efficiently on coverslips is presented, characterized, and compared to a conventional adsorption method. We studied the adhesion and maturation of primary cortical neurons with various morphological and functional approaches, including phase contrast microscopy, immunocytochemistry, scanning electron microscopy, patch clamp recordings, and calcium imaging. Results: We observed that several parameters of neuronal maturation are influenced by the substrate: neurons develop more dense and extended networks and synaptic activity is enhanced, when seeded on covalently bound PDL compared to adsorbed PDL. Discussion: Hence, we established reproducible and optimal conditions enhancing maturation of primary cortical neurons
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