ArticleMethodsX2023
Isolation of wheat thylakoids for protein analysis.
Article in MethodsX, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Thylakoids host a large number of proteins to confer photosynthesis and chemical biosynthesis essential for plant survival and growth. Successful isolation of high-quality thylakoids is the first step to studying the compositions and function of thylakoid protein and metabolites. Nevertheless, former studies isolated chloroplasts and thylakoids using a high-speed centrifuge with Percoll, which was expensive and unfriendly to the environment. The method presented here aims to establish a simple and inexpensive method to isolate high-quality thylakoids for protein analysis by utilizing sucrose instead of Percoll to reduce the cost and modify the centrifuge speed into the range usually used in labs.
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