ArticlePlant communications2024
Dissection of a rapidly evolving wheat resistance gene cluster by long-read genome sequencing accelerated the cloning of Pm69.
Article in Plant communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed, 84 citations in OpenAlex.
- Agronomic Performance and Dual Resistance Evaluation to Powdery Mildew and Stripe Rust in 660 Wheat Germplasm Lines.Plants (Basel, Switzerland) · 2026Article
- Identification of candidate gene for the wheat powdery mildew resistance gene Pm40 by bulked segregant analysis coupled with whole-genome sequencing (BSA-seq).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Characterization of Durum-Einkorn Amphiploids for Introgression of Powdery Mildew Resistance from Einkorn into Common Wheat.Pathogens (Basel, Switzerland) · 2026Article
- Wheat's war against stripe rust: Integrating host immunity, genomics and breeding for durable resistance.The plant genome · 2026Review
- WheatRPred: deep learning framework for predicting disease resistance protein in wheat and its wild relatives.Journal, genetic engineering & biotechnology · 2026Article
- The I gene defines a dynamic NLR cluster conferring broad potyvirus resistance in common bean.Nature communications · 2026Article
- Fine mapping of the TdHLS gene controlling hairy leaf sheath from wild emmer wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Physical Mapping of a Powdery Mildew Resistance Gene in Chromosome 6St from Wheat-Plants (Basel, Switzerland) · 2026Article
- Fine mapping and candidate gene dissection of the wheat powdery mildew resistance gene PmPBDH.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Pm37 as a susceptible Sr22 allele confers resistance to wheat powdery mildew and leaf rust.Nature communications · 2026Article
- Refined chloroplast annotations, repeat profiles, and phylogenomic evidence reveal maternal lineage shifts and independent evolution in the Triticum-Aegilops complex.BMC plant biology · 2026Article
- Pan-NLRome ofFrontiers in plant science · 2026Article
- Genetic resource optimization: a core collection of tetraploid wheat from 2,254 NBRP-wheat accessions.BMC plant biology · 2025Article
- Characterization and genetic dissection of the powdery mildew resistance in cultivated emmer wheat accession L424.BMC plant biology · 2025Article
- Paired NLRs originated from Triticum dicoccoides coordinately confer resistance to powdery mildew in wheat.Nature communications · 2025Article
- Two complementary NLRs from wild emmer wheat confer powdery mildew resistance.Nature communications · 2025Article
- An NLR pair in the Pm68 locus confers powdery mildew resistance in durum and common wheat.Nature communications · 2025Article
- Identification and characterization of a novel powdery mildew resistance gene PmCWI45575 in wild emmer wheat.BMC plant biology · 2025Article
- Characterization and mapping of a rust resistance locus in the common bean landrace G19833.G3 (Bethesda, Md.) · 2025Article
- Genetic Basis Identification of aPlants (Basel, Switzerland) · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
19 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene cloning in repeat-rich polyploid genomes remains challenging. Here, we describe a strategy for overcoming major bottlenecks in cloning of the powdery mildew resistance gene (R-gene) Pm69 derived from tetraploid wild emmer wheat. A conventional positional cloning approach was not effective owing to suppressed recombination. Chromosome sorting was compromised by insufficient purity. A Pm69 physical map, constructed by assembling Oxford Nanopore Technology (ONT) long-read genome sequences, revealed a rapidly evolving nucleotide-binding leucine-rich repeat (NLR) R-gene cluster with structural variations. A single candidate NLR was identified by anchoring RNA sequencing reads from susceptible mutants to ONT contigs and was validated by virus-induced gene silencing. Pm69 is likely a newly evolved NLR and was discovered in only one location across the wild emmer wheat distribution range in Israel. Pm69 was successfully introgressed into cultivated wheat, and a diagnostic molecular marker was used to accelerate its deployment and pyramiding with other R-genes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.