Evidence map›Paper›PMID 37415245›Full record

ArticleBMC research notes2023

An efficient method for protoplast-mediated production of transformed castor bean (Ricinus communis) lines.

Paula Figueroa-Varela, Danna Susunaga-Gómez, Catalina Restrepo-Osorio, Carsten Harms, Diego Villanueva-Mejía

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Genome Editing Approaches in Flax (International journal of molecular sciences · 2026
    Review
  2. Article
  3. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Paula Figueroa-VarelaCIBIOP Research Group, School of Applied Sciences and Engineering, EAFIT University, Medellín, Colombia.
Danna Susunaga-GómezCIBIOP Research Group, School of Applied Sciences and Engineering, EAFIT University, Medellín, Colombia.
Catalina Restrepo-OsorioCIBIOP Research Group, School of Applied Sciences and Engineering, EAFIT University, Medellín, Colombia.
Carsten HarmsBremerhaven Institute for Applied Molecular Biology, University of Applied Sciences Bremerhaven, Bremerhaven, Germany.
Diego Villanueva-MejíaCIBIOP Research Group, School of Applied Sciences and Engineering, EAFIT University, Medellín, Colombia. dvillanu@eafit.edu.co.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThe purpose of this study was to develop a method for the isolation, culture, and PEG-mediated protoplast transfection from leaves of in vitro-grown plants of Ricinus communis.

resultsFactors such as the enzymatic composition and the incubation time were evaluated. The enzymatic solution, containing 1.6% Cellulase-R10 and 0.8% Macerozyme-R10, with 16 h of incubation, was the best condition to achieve a high protoplast yield (481.16 × 10

Indexed as

Ricinus communisPlant LeavesProtoplastsRicinusTransfectionCellulaseMacerozymePEG-mediated transfectionProtoplasts

Identifiers

PMID37415245
PMCPMC10327310

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.