ArticleScientific data2023
Single cell transcriptome sequencing of stimulated and frozen human peripheral blood mononuclear cells.
Article in Scientific data, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- A map of intra- and intercellular immune responses across diverse in vitro stimuli and inflammatory disease.Genome medicine · 2026Article
- Direct detection of rare circulating tumor cells in peripheral blood mononuclear cells by scRNA seq: Spike-in strategy based feasibility study.The journal of liquid biopsy · 2026Article
- Advances in Single-Cell Transcriptomics for Livestock Health.Veterinary sciences · 2026Review
- Article
- Parallel single-cell host immune profiling and pathogen genomic characterization inFrontiers in cellular and infection microbiology · 2026Observational
- Blood transcriptomic profiling reveals gene expression alterations in patients with SFTS-associated encephalitis.Microbiology spectrum · 2025Article
- Microbiota humanization drives human-like metabolic and immune transcriptomic shifts in pigs.iMetaOmics · 2025Article
- Single-cell transcriptomics uncovers key immune drivers of vaccine efficacy in cattle.BMC genomics · 2025Article
- CryoSCAPE: Scalable immune profiling using cryopreserved whole blood for multi-omic single cell and functional assays.Journal of translational medicine · 2025Article
- Amplification of select autonomous HERV loci and surrounding host gene transcription in monocytes from patients with post-acute sequelae of COVID-19.Frontiers in immunology · 2025Article
- Single-cell RNA-sequencing of peripheral blood mononuclear cells reveals the transcriptome profile ofFrontiers in cellular and infection microbiology · 2025Article
- cfDiffusion: diffusion-based efficient generation of high quality scRNA-seq data with classifier-free guidance.Briefings in bioinformatics · 2024Article
- Shared and unique patterns of autonomous human endogenous retrovirus loci transcriptomes in CD14 + monocytes from individuals with physical trauma or infection with COVID-19.Retrovirology · 2024Article
- Single-cell sequencing: Current applications in various tuberculosis specimen types.Cell proliferation · 2024Review
- Single-cell RNA-sequencing reveals a profound immune cell response in human cytomegalovirus-infected humanized mice.Virologica Sinica · 2024Article
- Comparative proteomic profiling of the ovine and human PBMC inflammatory response.Scientific reports · 2024Article
- Characterization of the Immune Microenvironment and Identification of Biomarkers in Chronic Rhinosinusitis with Nasal Polyps Using Single-Cell RNA Sequencing and Transcriptome Analysis.Journal of inflammation research · 2024Article
- Th2 cell clonal expansion at diagnosis in human type 1 diabetes.Clinical immunology (Orlando, Fla.) · 2023Article
- Single cell transcriptome sequencing of stimulated and frozen human peripheral blood mononuclear cells.Scientific data · 2023Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Peripheral blood mononuclear cells (PBMCs) are blood cells that are a critical part of the immune system used to fight off infection, defending our bodies from harmful pathogens. In biomedical research, PBMCs are commonly used to study global immune response to disease outbreak and progression, pathogen infections, for vaccine development and a multitude of other clinical applications. Over the past few years, the revolution in single-cell RNA sequencing (scRNA-seq) has enabled an unbiased quantification of gene expression in thousands of individual cells, which provides a more efficient tool to decipher the immune system in human diseases. In this work, we generate scRNA-seq data from human PBMCs at high sequencing depth (>100,000 reads/cell) for more than 30,000 cells, in resting, stimulated, fresh and frozen conditions. The data generated can be used for benchmarking batch correction and data integration methods, and to study the effect of freezing-thawing cycles on the quality of immune cell populations and their transcriptomic profiles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.