Evidence map›Paper›PMID 37407494›Full record

ArticleThe Journal of veterinary medical science2023

Formation of free oocysts in Anopheles mosquitoes injected with Plasmodium ookinetes.

Asako Haraguchi, Makoto Takano, Jun Hakozaki, Kazuhiko Nakayama, Sakure Nakamura, Yasunaga Yoshikawa, Shinya Fukumoto, Kodai Kusakisako, Hiromi Ikadai

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of veterinary medical science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.4field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Asako HaraguchiLaboratory of Veterinary Parasitology, School of Veterinary Medicine, Kitasato University, Aomori, Japan.
Makoto TakanoLaboratory of Veterinary Parasitology, School of Veterinary Medicine, Kitasato University, Aomori, Japan.
Jun HakozakiLaboratory of Veterinary Parasitology, School of Veterinary Medicine, Kitasato University, Aomori, Japan.
Kazuhiko NakayamaLaboratory of Veterinary Parasitology, School of Veterinary Medicine, Kitasato University, Aomori, Japan.
Sakure NakamuraLaboratory of Veterinary Parasitology, School of Veterinary Medicine, Kitasato University, Aomori, Japan.
Yasunaga YoshikawaLaboratory of Veterinary Biochemistry, School of Veterinary Medicine, Kitasato University, Aomori, Japan.
Shinya FukumotoNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Kodai KusakisakoLaboratory of Veterinary Parasitology, School of Veterinary Medicine, Kitasato University, Aomori, Japan.
Hiromi IkadaiLaboratory of Veterinary Parasitology, School of Veterinary Medicine, Kitasato University, Aomori, Japan.
Kitasato University · JPObihiro University of Agriculture and Veterinary Medicine · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Malaria needs new strategies for its control. Plasmodium spp., the causative agent of malaria, is transmitted by mosquitoes. These parasites develop into oocysts and sporozoites in the body of the mosquitoes. A deeper understanding of oocysts that produce the infectious form of the parasite, sporozoites, can facilitate the development of novel countermeasures. However, the isolation of Plasmodium oocysts is challenging as these are formed between midgut epithelial cells and basal lamina after gametocytes enter the mosquito's body through blood feeding. Further research on oocysts has been impeded by issues related to oocyst isolation. Therefore, in this study, we injected Plasmodium into mosquitoes-an artificial and unique method-and aimed to clarify how oocysts were formed in mosquitoes after Plasmodium injection and whether free oocysts were formed from the mosquito tissue. Plasmodium berghei (ANKA strain) ookinetes cultured in vitro were injected into the thoracic body cavity (hemocoel) of female and male Anopheles stephensi mosquitoes. Oocysts were formed in the body of female and male mosquitoes at 14 days post injection. In addition, oocysts formed as a result of injection developed into sporozoites, which were infectious to mice. These findings suggest that P. berghei can complete its developmental stage in mosquitoes by injection. Some of the oocysts formed were free from mosquito tissue, and it was possible to collect oocysts with minimal contamination of mosquito tissue. These free oocysts can be used for investigating oocyst proteins and metabolism.

Indexed as

AnophelesMalariaAnimalsFemaleMaleMiceOocystsPlasmodium bergheiAnophelesfree oocystPlasmodium

Identifiers

PMID37407494
PMCPMC10539829
OpenAlexW4383113875

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.