Evidence map›Paper›PMID 37405487›Full record

ArticleActa crystallographica. Section F, Structural biology communications2023

Crystal structure of CmnB involved in the biosynthesis of the nonproteinogenic amino acid L-2,3-diaminopropionic acid.

Shu Ing Toh, Chieh Ling Lo, Chin Yuan Chang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Shu Ing TohDepartment of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu 30010, Taiwan.
Chieh Ling LoDepartment of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu 30010, Taiwan.
Chin Yuan ChangDepartment of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu 30010, Taiwan.ORCID 0000-0001-7314-3967
National Yang Ming Chiao Tung University · TW

Funding

Ministry of Science and Technology, Taiwan 110-2113-M-A49-026-MY3Ministry of Science and Technology, Taiwan 111-2113-M-002-025
6 · The paper itself

Abstract

L-2,3-Diaminopropionic acid (L-Dap) is a nonproteinogenic amino acid that plays as an important role as a building block in the biosynthesis of several natural products, including capreomycin, viomycin, zwittermicin, staphyloferrin and dapdiamide. A previous study reported that CmnB and CmnK are two enzymes that are involved in the formation of L-Dap in the biosynthesis of capreomycin. CmnB catalyzes the condensation reaction of O-phospho-L-serine and L-glutamic acid to generate N-(1-amino-1-carboxyl-2-ethyl)glutamic acid, which subsequently undergoes oxidative hydrolysis via CmnK to generate the product L-Dap. Here, the crystal structure of CmnB in complex with the reaction intermediate PLP-α-aminoacrylate is reported at 2.2 Å resolution. Notably, CmnB is the second known example of a PLP-dependent enzyme that forms a monomeric structure in crystal packing. The crystal structure of CmnB also provides insights into the catalytic mechanism of the enzyme and supports the biosynthetic pathway of L-Dap reported in previous studies.

Indexed as

Amino AcidsCapreomycinbeta-AlanineCrystallography, X-RayGlutamic Acid2,3-diaminopropionic acidAmino Acidsbeta-AlanineCapreomycinGlutamic Acidcapreomycin biosynthesisCmnBl-2,3-diaminopropionic acidl-DapPLP-dependent enzymes

Identifiers

PMID37405487
PMCPMC10327575
OpenAlexW4383186767

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.