Evidence map›Paper›PMID 37405466›Full record

ArticleLung2023

Whole Genome Sequencing in Advanced Lung Cancer can be Performed Using Diff-Quik Cytology Smears Derived from Endobronchial Ultrasound, Transbronchial Needle Aspiration (EBUS TBNA).

David Fielding, Andrew J Dalley, Mahendra Singh, Lakshmy Nandakumar, Vanessa Lakis, Haarika Chittoory, David Fairbairn, Kaltin Ferguson, Farzad Bashirzadeh, Michael Bint and 9 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Lung, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.0field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 5 institutions in 1 country.

David FieldingDepartment of Thoracic Medicine, The Royal Brisbane & Women's Hospital, Brisbane, Australia. David.fielding@health.qld.gov.au.
Andrew J DalleyFaculty of Medicine, UQ Centre for Clinical Research, The University of Queensland, Brisbane, Australia.
Mahendra SinghFaculty of Medicine, UQ Centre for Clinical Research, The University of Queensland, Brisbane, Australia.
Lakshmy NandakumarPathology Queensland, The Royal Brisbane & Women's Hospital, Brisbane, Australia.
Vanessa LakisQIMR Berghofer Medical Research Institute, Brisbane, Australia.
Haarika ChittooryFaculty of Medicine, UQ Centre for Clinical Research, The University of Queensland, Brisbane, Australia.
David FairbairnPathology Queensland, The Royal Brisbane & Women's Hospital, Brisbane, Australia.
Kaltin FergusonFaculty of Medicine, UQ Centre for Clinical Research, The University of Queensland, Brisbane, Australia.
Farzad BashirzadehDepartment of Thoracic Medicine, The Royal Brisbane & Women's Hospital, Brisbane, Australia.
Michael BintDepartment of Thoracic Medicine, Sunshine Coast University Hospital, Birtinya, Australia.
Carl PahoffDepartment of Respiratory Medicine, Gold Coast University Hospital, Southport, Australia.
Jung Hwa SonDepartment of Thoracic Medicine, The Royal Brisbane & Women's Hospital, Brisbane, Australia.
Alan HodgsonPathology Queensland, The Royal Brisbane & Women's Hospital, Brisbane, Australia.
John V PearsonQIMR Berghofer Medical Research Institute, Brisbane, Australia.
Nicola WaddellQIMR Berghofer Medical Research Institute, Brisbane, Australia.
Sunil R LakhaniFaculty of Medicine, UQ Centre for Clinical Research, The University of Queensland, Brisbane, Australia.
Gunter Hartel *QIMR Berghofer Medical Research Institute, Brisbane, Australia.
Katia Nones *QIMR Berghofer Medical Research Institute, Brisbane, Australia.
Peter T Simpson *Faculty of Medicine, UQ Centre for Clinical Research, The University of Queensland, Brisbane, Australia.
The University of Queensland · AURoyal Brisbane and Women's Hospital · AUQIMR Berghofer Medical Research Institute · AUGold Coast Hospital · AUSunshine Coast University Hospital · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionMaximising alternative sample types for genomics in advanced lung cancer is important because bronchoscopic samples may sometimes be insufficient for this purpose. Further, the clinical applications of comprehensive molecular analysis such as whole genome sequencing (WGS) are rapidly developing. Diff-Quik cytology smears from EBUS TBNA is an alternative source of DNA, but its feasibility for WGS has not been previously demonstrated.

methodsDiff-Quik smears were collected along with research cell pellets.

resultsTumour content of smears were compared to research cell pellets from 42 patients, which showed good correlation (Spearman correlation 0.85, P < 0.0001). A subset of eight smears underwent WGS, which presented similar mutation profiles to WGS of the matched cell pellet. DNA yield was predicted using a regression equation of the smears cytology features, which correctly predicted DNA yield > 1500 ng in 7 out of 8 smears.

conclusionsWGS of commonly collected Diff-Quik slides is feasible and their DNA yield can be predicted.

Indexed as

Lung NeoplasmsAzure StainsBiopsy, Fine-NeedleBronchoscopyEndoscopic Ultrasound-Guided Fine Needle AspirationEndosonographyHumansLymph NodesMethylene BlueWhole Genome SequencingXanthenesAzure StainsDiff QuikMethylene BlueXanthenesCytologyEndobronchial ultrasound–guided transbronchial needle aspiration (EBUS TBNA)Lung cancerMolecular diagnosticsWhole genome sequencing

Identifiers

PMID37405466
PMCPMC10444633
OpenAlexW4383187626

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.