ReviewApplied microbiology and biotechnology2023
Heterologous protein production in filamentous fungi.
Review in Applied microbiology and biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 76 citations in OpenAlex.
- Profiling heterologous 3' terminator-dependent expression variation inSynthetic and systems biotechnology · 2026Article
- High-throughput droplet screening of filamentous fungi on solid substrates.RSC chemical biology · 2026Article
- Semi-automated strain engineering and screening in the thermophilic fungusApplied and environmental microbiology · 2026Article
- Barcoded mutant library enables high-throughput functional genomics in a filamentous fungus.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Production of Recombinant Hemoglobin inFoods (Basel, Switzerland) · 2026Article
- Heterologous Systems in the Functional Characterization of Proteins from Phytopathogenic Fungi: A Systematic Review.Molecular biotechnology · 2026Article
- Two-Step Engineering of Food-GradeJournal of fungi (Basel, Switzerland) · 2026Article
- Effect of Different Signal Peptides on the Expression of Glucoamylase fromJournal of fungi (Basel, Switzerland) · 2026Article
- RoCi-a single-step multi-copy integration system based on rolling-circle replication.Nucleic acids research · 2026Article
- Mycoproteins and Beyond: Biotechnological Advances in Filamentous Fungal Biomass.Mini reviews in medicinal chemistry · 2026Review
- Multi-omics analysis reveals the carbohydrate-active enzyme repertoire involved in degrading complex arabinoxylans in Penicillium parvum 4-14.Microbial cell factories · 2025Article
- Characterization and Development of Salt-Resistant Aspergillus oryzae for Marine-Derived Enzyme Expression.Applied biochemistry and biotechnology · 2025Article
- Fungal Innovations-Advancing Sustainable Materials, Genetics, and Applications for Industry.Journal of fungi (Basel, Switzerland) · 2025Review
- Establishment of an efficient Agrobacterium tumefaciens-mediated transformation system for an Armillaria species, a host of the fully mycoheterotrophic plant Gastrodia elata.Folia microbiologica · 2025Article
- Article
- The Transformation and Protein Expression of the Edible MushroomJournal of fungi (Basel, Switzerland) · 2025Article
- Advances in Microbial Alkaline Proteases: Addressing Industrial Bottlenecks Through Genetic and Enzyme Engineering.Applied biochemistry and biotechnology · 2025Review
- Recent Advances in Heterologous Protein Expression and Natural Product Synthesis byJournal of fungi (Basel, Switzerland) · 2025Review
- Investigating the Effect of Membrane Composition on the Selective Ammonium Transport of Escherichia coli AmtB Membrane Proteins.ACS applied engineering materials · 2025Article
- Development of a CRISPR/Cas9 RNP-mediated genetic engineering system in Paecilomyces variotii.Journal of microbiology (Seoul, Korea) · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
Filamentous fungi are able to produce a wide range of valuable proteins and enzymes for many industrial applications. Recent advances in fungal genomics and experimental technologies are rapidly changing the approaches for the development and use of filamentous fungi as hosts for the production of both homologous and heterologous proteins. In this review, we highlight the benefits and challenges of using filamentous fungi for the production of heterologous proteins. We review various techniques commonly employed to improve the heterologous protein production in filamentous fungi, such as strong and inducible promoters, codon optimization, more efficient signal peptides for secretion, carrier proteins, engineering of glycosylation sites, regulation of the unfolded protein response and endoplasmic reticulum associated protein degradation, optimization of the intracellular transport process, regulation of unconventional protein secretion, and construction of protease-deficient strains. KEY POINTS: • This review updates the knowledge on heterologous protein production in filamentous fungi. • Several fungal cell factories and potential candidates are discussed. • Insights into improving heterologous gene expression are given.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.