ArticleAnimal bioscience2023
Identification of heat shock protein70-2 and protamine-1 mRNA, proteins, and analyses of their association with fertility using frozen-thawed sperm in Madura bulls.
Article in Animal bioscience, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
9 citing papers in PubMed, 1 synthesis or guideline pooled it, 11 citations in OpenAlex.
- Association between sperm DNA fragmentation and fertility parameters in farm animals: a systematic review and meta-analysis.BMC veterinary research · 2025Pooled it
- From sperm to pregnancy: insights into paternal regulation of fertility divergence in cattle.The Journal of reproduction and development · 2026Review
- Age-related differences in sperm chromatin packaging and protein profiles based on SDS-PAGE in Bali bulls (Journal of advanced veterinary and animal research · 2026Article
- Unveiling novel insights into the role and characteristics of sperm heat shock protein 70 in indigenous Donggala bulls: implications for molecular-based motility markers.Archives animal breeding · 2026Article
- A-Kinase Anchor Protein 4 (proAKAP4): Protein Molecule-Based Fertility Marker of Indonesian Dairy Bull and Its Correlation With Frozen-Thawed Sperm Quality.Veterinary medicine international · 2025Article
- Reduction of post-thawed sperm quality of Kacang goat using the addition of epigallocatechin-3-gallate into the skim milk-egg yolk extender.Open veterinary journal · 2025Article
- Sperm HSP70: may not be an age-dependent gene but is associated with field fertility in Bali bulls (Animal reproduction · 2024Article
- Sperm Head Morphology Alterations Associated with Chromatin Instability and Lack of Protamine Abundance in Frozen-Thawed Sperm of Indonesian Local Bulls.Animals : an open access journal from MDPI · 2023Article
- A proteomic approach to identifying spermatozoa proteins in Indonesian native Madura bulls.Frontiers in veterinary science · 2023Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveThis study aims to identify heat shock protein70-2 (HSP70-2) and protamine-1 (PRM1) mRNA and protein in Madura bull sperm and demonstrate their relation as bull fertility biomarkers.
methodsThe Madura bull fertility rates were grouped based on the percentage of first service conception rate (%FSCR) as high fertility (HF) (79.04%; n = 4), and low fertility (LF) (65.84%; n = 4). mRNA of HSP70-2 and PRM1 with peptidylprolyl isomerase A (PPIA) as a housekeeping gene were determined by quantitative real-time polymerase chain reaction, while enzyme-linked immunoassay was used to measure protein abundance. In the post-thawed semen samples, sperm motility, viability, acrosome integrity, and sperm DNA fragmentation index were analyzed. Data analysis was performed on the measured parameters of semen quality, relative mRNA expression, and protein abundance of HSP70-2 and PRM1, among the bulls with various fertility levels (HF and LF) in a one-way analysis of variance analysis. The Pearson correlation was used to analyze the relationship between semen quality, mRNA, proteins, and fertility rate.
resultsRelative mRNA expression and protein abundance of HSP70-2 and PRM1 were detected and were found to be highly expressed in bulls with HF (p<0.05) and were associated with several parameters of semen quality.
conclusionHSP70-2 and PRM1 mRNA and protein molecules have great potential to serve as molecular markers for determining bull fertility.
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