ArticleChemical communications (Cambridge, England)2023
Proline-conditioning and chemically-programmed ice nucleation protects spheroids during cryopreservation.
Article in Chemical communications (Cambridge, England), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- Cryopreservation of Avian Erythrocytes Using Macromolecular Cryoprotectants.ACS polymers Au · 2026Article
- Induced Extracellular Ice Nucleation Protects Cocultured Spheroid Interior and Exterior during Cryopreservation.ACS biomaterials science & engineering · 2025Article
- Proline pre-conditioning of Jurkat cells improves recovery after cryopreservation.RSC medicinal chemistry · 2023Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Spheroids mimic 3-D tissue niches better than standard cell cultures. Cryopreserving spheroids, however, remains challenging as conventional cryoprotectants do not mitigate all damage mechanisms. Here chemically-programmed extracellular ice nucleation is used to prevent supercooling, alongside proline pre-conditioning, which are found to synergystically improve post-thaw recovery of spheroids. This validates the need to identify compounds and materials to address both biochemical and biophysical damage pathways beyond standard cryoprotectants.
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Registered trials
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