Evidence map›Paper›PMID 37401411›Full record

ArticleDevelopment (Cambridge, England)2023

Optogenetic control of Wnt signaling models cell-intrinsic embryogenic patterning using 2D human pluripotent stem cell culture.

Nicole A Repina, Hunter J Johnson, Xiaoping Bao, Joshua A Zimmermann, David A Joy, Shirley Z Bi, Ravi S Kane, David V Schaffer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Development (Cambridge, England), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Review
  6. Article
  7. Review
  8. Optogenetic Rescue Reveals Spatiotemporal Rules of Germ-Layer Patterning.bioRxiv : the preprint server for biology · 2025
    Article
  9. Article
  10. Review
  11. Article
  12. Article
  13. Review
  14. Review
  15. Article
  16. Review
  17. Rapid optogenetic clustering of a cytoplasmic BcLOV4 variant.bioRxiv : the preprint server for biology · 2023
    Article
  18. Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Nicole A RepinaDepartment of Bioengineering, University of California, Berkeley, CA 94720, USA.ORCID 0000-0003-2229-5084
Hunter J JohnsonDepartment of Bioengineering, University of California, Berkeley, CA 94720, USA.
Xiaoping BaoDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA.
Joshua A ZimmermannDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA.
David A JoyGraduate Program in Bioengineering, University of California, San Francisco and University of California, Berkeley, CA 94720, USA.
Shirley Z BiDepartment of Bioengineering, University of California, Berkeley, CA 94720, USA.
Ravi S KaneSchool of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
David V SchafferDepartment of Bioengineering, University of California, Berkeley, CA 94720, USA.ORCID 0000-0002-9625-0121

Funding

Optogenetic Characterization and Control of Stem Cell SignalingR01NS087253 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI KANE, RAVI S., SCHAFFER, DAVID V · 2014 to 2018
$1.7M
NINDS NIH HHS R01 NS087253
6 · The paper itself

Abstract

In embryonic stem cell (ESC) models for early development, spatially and temporally varying patterns of signaling and cell types emerge spontaneously. However, mechanistic insight into this dynamic self-organization is limited by a lack of methods for spatiotemporal control of signaling, and the relevance of signal dynamics and cell-to-cell variability to pattern emergence remains unknown. Here, we combine optogenetic stimulation, imaging and transcriptomic approaches to study self-organization of human ESCs (hESC) in two-dimensional (2D) culture. Morphogen dynamics were controlled via optogenetic activation of canonical Wnt/β-catenin signaling (optoWnt), which drove broad transcriptional changes and mesendoderm differentiation at high efficiency (>99% cells). When activated within cell subpopulations, optoWnt induced cell self-organization into distinct epithelial and mesenchymal domains, mediated by changes in cell migration, an epithelial to mesenchymal-like transition and TGFβ signaling. Furthermore, we demonstrate that such optogenetic control of cell subpopulations can be used to uncover signaling feedback mechanisms between neighboring cell types. These findings reveal that cell-to-cell variability in Wnt signaling is sufficient to generate tissue-scale patterning and establish a hESC model system for investigating feedback mechanisms relevant to early human embryogenesis.

Indexed as

Pluripotent Stem CellsWnt Signaling Pathwaybeta CateninCell DifferentiationEmbryonic Stem CellsHumansOptogeneticsbeta CateninDifferentiationHeterogeneityMorphogenesisOptogeneticsPatterningWnt signaling

Identifiers

PMID37401411
PMCPMC10399980

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.