ArticleAmerican journal of physiology. Lung cellular and molecular physiology2023
Optimizing anesthesia and delivery approaches for dosing into lungs of mice.
Article in American journal of physiology. Lung cellular and molecular physiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 13 citations in OpenAlex.
- Establishment of a chronic aspiration pneumonia mouse model using oropharyngeal aspiration of food suspension.Animal models and experimental medicine · 2026Article
- The COMBINE pneumonia model: a multicenter study to standardize a mouse pneumonia model withMicrobiology spectrum · 2026Article
- Dapagliflozin improves diabetic kidney disease by inhibiting ferroptosis through β-hydroxybutyrate production.Renal failure · 2025Article
- Optimization of intranasal bleomycin dose for effective pulmonary fibrosis induction in mice with minimal animal distress.BMC pulmonary medicine · 2025Article
- Spatial Transcriptomics to Study Virus-Host Interactions.Annual review of virology · 2025Review
- Improved Methodology for Liquid Delivery to the Mouse Lung: Intubation using a Consumer Otoscope.Journal of visualized experiments : JoVE · 2025Article
- Article
- Intravital imaging of pulmonary lymphatics in inflammation and metastatic cancer.The Journal of experimental medicine · 2025Article
- Anesthetic choice impacts mortality and bacterial clearance in a murine experimental pneumonia model.BMC infectious diseases · 2025Article
- Nrf2 Regulates Inflammation by Modulating Dendritic Cell-T Cell Crosstalk during Viral-Bacterial Superinfection.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- Comparative pathogenicity of influenza virus-induced pneumonia mouse model following intranasal and aerosolized intratracheal inoculation.Virology journal · 2024Article
- Long-lived lung megakaryocytes contribute to platelet recovery in thrombocytopenia models.The Journal of clinical investigation · 2024Article
- Intravital imaging of pulmonary lymphatics in inflammation and metastatic cancer.bioRxiv : the preprint server for biology · 2024Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Microbes, toxins, therapeutics, and cells are often instilled into lungs of mice to model diseases and test experimental interventions. Consistent pulmonary delivery is critical for experimental power and reproducibility, but we observed variation in outcomes between handlers using different anesthetic approaches for intranasal dosing in mice. We therefore used a radiotracer to quantify lung delivery after intranasal dosing under inhalational (isoflurane) versus injectable (ketamine/xylazine) anesthesia in C57BL/6 mice. We found that ketamine/xylazine anesthesia resulted in delivery of a greater proportion (52 ± 9%) of an intranasal dose to lungs relative to isoflurane anesthesia (30 ± 15%). This difference in pulmonary dose delivery altered key outcomes in models of viral and bacterial pneumonia, with mice anesthetized with ketamine/xylazine for intranasal infection with influenza A virus or
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