ArticlePlant biotechnology journal2023
A large-scale whole-exome sequencing mutant resource for functional genomics in wheat.
Article in Plant biotechnology journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Cysteine-rich receptor-like kinase and MADS-box co-regulation of programmed cell death drives apical spikelet degeneration in wheat.Plant physiology · 2026Article
- Unlocking wheat fertility: map-based cloning spotlights anther-specific TaAGO5c as a master modulator.The Plant journal : for cell and molecular biology · 2026Article
- The transcription factor TaWRKY58 coordinates growth and drought sensitivity in wheat by repressing TaLRR and TaBCS1.PLoS genetics · 2026Article
- A Forward Genetics Strategy for High-Throughput Gene Identification via Precise Image-Based Phenotyping of an Indexed EMS Mutant Library.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A simple method to efficiently generate structural variation in plants.PLoS genetics · 2025Article
- Cereal Cyst Nematode Effector HfVAP2 Targets and Stabilizes TaCAT3-A1 to Suppress Immune Responses for Parasitism in Wheat.Journal of agricultural and food chemistry · 2025Article
- Text mining reveals the increasing importance of genomic approaches in crop research and breeding.Molecular breeding : new strategies in plant improvement · 2025Review
- Genomics-driven discovery of superior alleles and genes for yellow rust resistance in wheat.Nature genetics · 2025Article
- Targeted seed EMS mutagenesis reveals a basic helix-loop-helix transcription factor underlying male sterility in sorghum.Genetics · 2025Article
- Identification of novel candidate gene associated with early heading on chromosome 5B in wheat mutantMolecular breeding : new strategies in plant improvement · 2025Article
- Characterization of wheat oligo-tiller mutant ot2 and fine mapping of the mutant gene Taot2.The plant genome · 2025Article
- Wheat TaNADPO Promotes Spot Blotch Resistance.Molecular plant pathology · 2025Article
- A TOPLESS-related gene regulates both male and female sterility in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Advancing radiation-induced mutant screening through high-throughput technology: a preliminary evaluation of mutant screening in Arabidopsis thaliana.Plant methods · 2025Article
- A gain-of-function mutation at the C-terminus of FT-D1 promotes heading by interacting with 14-3-3A and FDL6 in wheat.Plant biotechnology journal · 2025Article
- Creating largeFrontiers in plant science · 2025Article
- Mapping of dwarfing gene and identification of mutant allele on plant height in wheat.Molecular breeding : new strategies in plant improvement · 2024Article
- Mutations in wheat TaAPA2 gene result in pleiotropic effects on plant architecture.Science China. Life sciences · 2024Article
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15 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Hexaploid wheat (Triticum aestivum), a major staple crop, has a remarkably large genome of ~14.4 Gb (containing 106 913 high-confidence [HC] and 159 840 low-confidence [LC] genes in the Chinese Spring v2.1 reference genome), which poses a major challenge for functional genomics studies. To overcome this hurdle, we performed whole-exome sequencing to generate a nearly saturated wheat mutant database containing 18 025 209 mutations induced by ethyl methanesulfonate (EMS), carbon (C)-ion beams, or γ-ray mutagenesis. This database contains an average of 47.1 mutations per kb in each gene-coding sequence: the potential functional mutations were predicted to cover 96.7% of HC genes and 70.5% of LC genes. Comparative analysis of mutations induced by EMS, γ-rays, or C-ion beam irradiation revealed that γ-ray and C-ion beam mutagenesis induced a more diverse array of variations than EMS, including large-fragment deletions, small insertions/deletions, and various non-synonymous single nucleotide polymorphisms. As a test case, we combined mutation analysis with phenotypic screening and rapidly mapped the candidate gene responsible for the phenotype of a yellow-green leaf mutant to a 2.8-Mb chromosomal region. Furthermore, a proof-of-concept reverse genetics study revealed that mutations in gibberellic acid biosynthesis and signalling genes could be associated with negative impacts on plant height. Finally, we built a publically available database of these mutations with the corresponding germplasm (seed stock) repository to facilitate advanced functional genomics studies in wheat for the broad plant research community.
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