Evidence map›Paper›PMID 37400744›Full record

ArticleJournal of experimental orthopaedics2023

Development and characterization of an intra-articular fracture mediated model of post-traumatic osteoarthritis.

Michael S Valerio, William A Pace, Connor P Dolan, Jorge B Edwards, Naveena B Janakiram, Benjamin K Potter, Christopher L Dearth, Stephen M Goldman

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of experimental orthopaedics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Michael S ValerioResearch and Surveillance Division DoD-VA Extremity Trauma and Amputation Center of Excellence, Bethesda, USA.ORCID http://orcid.org/0000-0003-1261-7078
William A PaceDepartment of Biomedical Engineering, Vanderbilt University, Nashville, USA.ORCID http://orcid.org/0000-0003-1877-3725
Connor P DolanResearch and Surveillance Division DoD-VA Extremity Trauma and Amputation Center of Excellence, Bethesda, USA.ORCID http://orcid.org/0000-0001-7002-9341
Jorge B EdwardsResearch and Surveillance Division DoD-VA Extremity Trauma and Amputation Center of Excellence, Bethesda, USA.
Naveena B JanakiramResearch and Surveillance Division DoD-VA Extremity Trauma and Amputation Center of Excellence, Bethesda, USA.ORCID http://orcid.org/0000-0002-0138-4654
Benjamin K PotterDepartment of Surgery, Uniformed Services University of the Health Sciences-Walter Reed National Military Medical Center, Bethesda, USA.ORCID http://orcid.org/0000-0002-8771-0317
Christopher L DearthResearch and Surveillance Division DoD-VA Extremity Trauma and Amputation Center of Excellence, Bethesda, USA.ORCID http://orcid.org/0000-0003-3701-0950
Stephen M GoldmanResearch and Surveillance Division DoD-VA Extremity Trauma and Amputation Center of Excellence, Bethesda, USA. stephen.m.goldman5.civ@mail.mil.ORCID http://orcid.org/0000-0002-2244-1443
Uniformed Services University of the Health Sciences · USHenry M. Jackson Foundation · USVanderbilt University · US

Funding

Medical Research and Materiel Command W81XWH1910201Orthopaedic Trauma Association 2819
6 · The paper itself

Abstract

purposeThis study aimed to develop and characterize a closed intra-articular fracture (IAF) mediated post-traumatic osteoarthritis (PTOA) model in rats to serve as a testbed for putative disease modifying interventions.

methodsMale rats were subject to a 0 Joule (J), 1 J, 3 J, or 5 J blunt-force impact to the lateral aspect of the knee and allowed to heal for 14 and 56 days. Micro-CT was performed at time of injury and at the specified endpoints to assess bone morphometry and bone mineral density measurements. Cytokines and osteochondral degradation markers were assayed from serum and synovial fluid via immunoassays. Histopathological analyses were performed on decalcified tissues and assessed for evidence of osteochondral degradation.

resultsHigh-energy (5 J) blunt impacts consistently induced IAF to the proximal tibia, distal femur, or both while lower energy (1 J and 3 J) impacts did not. CCL2 was found to be elevated in the synovial fluid of rats with IAF at both 14- and 56-days post-injury while COMP and NTX-1 were upregulated chronically relative to sham controls. Histological analysis showed increased immune cell infiltration, increased osteoclasts and osteochondral degradation with IAF relative to sham.

conclusionBased on results from the current study, our data indicates that a 5 J blunt-forced impact adequately and consistently induces hallmark osteoarthritic changes to the articular surface and subchondral bone at 56 days after IAF. Marked development of PTOA pathobiology suggest this model will provide a robust testbed for screening putative disease modifying interventions that might be translated to the clinic for militarily relevant, high-energy joint injuries.

Indexed as

Animal ModelsComminuted FracturesMilitary PersonnelMusculoskeletal SystemRegenerative MedicineTrauma

Identifiers

PMID37400744
PMCPMC10317931
OpenAlexW4383058965

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.