ReviewNature aging2023
Spatial mapping of cellular senescence: emerging challenges and opportunities.
Open access · greenAbstract readReview
In one paragraph
Review in Nature aging, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers, 1 of them a synthesis that pooled it.
0numbers the graph read from it
0cells of the map it votes in
89citing papers in PubMed, 1 pooled it
19.1field-weighted citation impact, top 1% of its field
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
89 citing papers in PubMed, 1 synthesis or guideline pooled it, 109 citations in OpenAlex.
- Cross-expression meta-analysis of mouse brain slices reveals coordinated gene expression across spatially adjacent cells.Genome biology · 2025Pooled it
- Mechanistic reprogramming of the mtDNA-cGAS-STING axis in cellular senescence: from mitochondrial homeostatic disruption to inflammatory and immune outcomes.Biogerontology · 2026Review
- Menopausal timing and senescent-immune coupling in age-related lobular involution of the human breast: a longitudinal cohort study.EBioMedicine · 2026Article
- Halo: a pretrained model for whole-cell segmentation from nuclei images in spatial transcriptomics.Briefings in bioinformatics · 2026Article
- SenFlag gene signature identifies senescent cells in mouse and human tissues through a conserved core transcriptional program.The EMBO journal · 2026Article
- Tumor cells escape from therapy-induced senescence: from molecular mechanisms to targeted intervention.Biology direct · 2026Review
- Fueling the fire: metabolic dysfunction and senescence as drivers of lung aging and disease.Physiological reviews · 2026Review
- Cellular senescence as a therapeutic target for aging intervention.Biomedical journal · 2026Review
- Plasma proteomics reveals divergent sex-specific senescence and bone biology signatures across neurodegenerative diseases.bioRxiv : the preprint server for biology · 2026Article
- Review
- Fluorescence-Guided Spatial Mapping of p21-Expressing Senescent Cells in Aged Liver at Single-Cell Resolution.Biomolecules · 2026Article
- Chronic Kidney Disease and Cellular Senescence.International journal of molecular sciences · 2026Review
- Review
- iCLAP: an innovative method for integrable co-detection of low-abundance antigens with high-plex immunostaining.Nature communications · 2026Article
- SenCat: Cataloging human cell senescence through multiomic profiling of multiple senescent primary cell types.bioRxiv : the preprint server for biology · 2026Article
- The Sixth Annual Symposium of the Midwest Aging Consortium.The journals of gerontology. Series A, Biological sciences and medical sciences · 2026Article
- Therapy-induced senescence in breast cancer and cardiovascular disease: a double-edged biological process.Frontiers in cardiovascular medicine · 2026Review
- Spatiotemporal decoding of skin biology: development, aging, disease, and regeneration.Burns & trauma · 2026Article
- Integrating senotherapeutics into transplantation: liver reconditioning in an aging donor pool.Transplant international : official journal of the European Society for Organ Transplantation · 2026Review
- Decoding the tumor-aging axis: from bench to clinical.Frontiers in immunology · 2026Review
29 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
45 authors at 17 institutions in 2 countries.
Aditi U GurkarAging Institute, University of Pittsburgh School of Medicine/UPMC and Division of Pulmonary, Allergy and Critical Care Medicine, Allergy, Critical Care and Sleep Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.ORCID 0000-0003-4338-0624
Akos A GerencserBuck Institute for Research on Aging, Novato, CA, USA.
Ana L MoraDorothy M. Davis Heart and Lung Research Institute, Division of Pulmonary, Critical Care and Sleep Medicine, Department of Internal Medicine, the Ohio State University, Columbus, OH, USA.
Andrew C NelsonDepartment of Laboratory Medicine and Pathology, Department of Biochemistry, Molecular Biology and Biophysics, Department of Neuroscience and Institute on the Biology of Aging and Metabolism, Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, MN, USA.
Anru R ZhangDivision of Pulmonary, Allergy, and Critical Care Medicine, Department of Medicine and Department of Biostatistics and Bioinformatics, Duke University School of Medicine, Durham, NC, USA.
Anthony B LagnadoDepartment of Physiology and Biomedical Engineering, Robert and Arlene Kogod Center on Aging, Mayo Clinic, Rochester, MN, USA.
Archibald EnninfulDepartment of Biomedical Engineering, Yale University, New Haven, CT, USA.
Christopher BenzBuck Institute for Research on Aging, Novato, CA, USA.
David FurmanBuck Institute for Research on Aging, Novato, CA, USA.
Delphine BeaulieuAging Institute, University of Pittsburgh School of Medicine/UPMC and Division of Pulmonary, Allergy and Critical Care Medicine, Allergy, Critical Care and Sleep Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Diana JurkDepartment of Physiology and Biomedical Engineering, Robert and Arlene Kogod Center on Aging, Mayo Clinic, Rochester, MN, USA.ORCID 0000-0003-4486-0857
Elizabeth L ThompsonDepartment of Laboratory Medicine and Pathology, Department of Biochemistry, Molecular Biology and Biophysics, Department of Neuroscience and Institute on the Biology of Aging and Metabolism, Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, MN, USA.ORCID 0000-0001-8111-8382
Fei WuBuck Institute for Research on Aging, Novato, CA, USA.ORCID 0000-0002-8353-7676
Fernanda RodriguezDepartment of Laboratory Medicine and Pathology, Department of Biochemistry, Molecular Biology and Biophysics, Department of Neuroscience and Institute on the Biology of Aging and Metabolism, Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, MN, USA.ORCID 0000-0001-8504-2194
Grant BarthelDepartment of Laboratory Medicine and Pathology, Department of Biochemistry, Molecular Biology and Biophysics, Department of Neuroscience and Institute on the Biology of Aging and Metabolism, Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, MN, USA.ORCID 0000-0003-1238-7540
Hao ChenComputational Biology Department, School of Computer Science, Carnegie Mellon University, Pittsburgh, PA, USA.ORCID 0000-0002-3424-835X
Hemali PhatnaniColumbia University Irving Medical Center and New York Genome Center, Columbia University, New York, NY, USA.
Indra HeckenbachBuck Institute for Research on Aging, Novato, CA, USA.
Jeffrey H ChuangThe Jackson Laboratory for Genomic Medicine, Farmington, CT, USA.ORCID 0000-0002-3298-2358
Jeremy HorrellDepartment of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI, USA.
Joana PetrescuColumbia University Irving Medical Center and New York Genome Center, Columbia University, New York, NY, USA.
Jonathan K AlderAging Institute, University of Pittsburgh School of Medicine/UPMC and Division of Pulmonary, Allergy and Critical Care Medicine, Allergy, Critical Care and Sleep Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Jun Hee LeeDepartment of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI, USA.ORCID 0000-0002-2200-6011
Laura J NiedernhoferDepartment of Laboratory Medicine and Pathology, Department of Biochemistry, Molecular Biology and Biophysics, Department of Neuroscience and Institute on the Biology of Aging and Metabolism, Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, MN, USA.ORCID 0000-0002-1074-1385
Manoj KumarDepartment of Radiology, Molecular Imaging Program at Stanford, Stanford University, Stanford, CA, USA.ORCID 0000-0001-8202-2913
Melanie KönigshoffAging Institute, University of Pittsburgh School of Medicine/UPMC and Division of Pulmonary, Allergy and Critical Care Medicine, Allergy, Critical Care and Sleep Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Marta BuenoAging Institute, University of Pittsburgh School of Medicine/UPMC and Division of Pulmonary, Allergy and Critical Care Medicine, Allergy, Critical Care and Sleep Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.ORCID 0000-0001-7140-9899
Miiko SokkaDepartment of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI, USA.
Morten Scheibye-KnudsenBuck Institute for Research on Aging, Novato, CA, USA.ORCID 0000-0002-6637-1280
Nicola NerettiDepartment of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI, USA.
Oliver EickelbergAging Institute, University of Pittsburgh School of Medicine/UPMC and Division of Pulmonary, Allergy and Critical Care Medicine, Allergy, Critical Care and Sleep Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Peter D AdamsSanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, USA.ORCID 0000-0002-0684-1770
Qianjiang HuAging Institute, University of Pittsburgh School of Medicine/UPMC and Division of Pulmonary, Allergy and Critical Care Medicine, Allergy, Critical Care and Sleep Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.ORCID 0000-0003-1069-3181
Quan ZhuUniversity of California, San Diego, CA, USA.
Rebecca A PorrittSanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, USA.ORCID 0000-0002-0973-5092
Runze DongDepartment of Biochemistry, Institute for Protein Design and Graduate Program in Biological Physics, Structure and Design, University of Washington, Seattle, WA, USA.
Samuel PetersDepartment of Laboratory Medicine and Pathology, Department of Biochemistry, Molecular Biology and Biophysics, Department of Neuroscience and Institute on the Biology of Aging and Metabolism, Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, MN, USA.
Stella VictorelliDepartment of Physiology and Biomedical Engineering, Robert and Arlene Kogod Center on Aging, Mayo Clinic, Rochester, MN, USA.
Thomas PengoDepartment of Laboratory Medicine and Pathology, Department of Biochemistry, Molecular Biology and Biophysics, Department of Neuroscience and Institute on the Biology of Aging and Metabolism, Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, MN, USA.
Timur KhaliullinDorothy M. Davis Heart and Lung Research Institute, Division of Pulmonary, Critical Care and Sleep Medicine, Department of Internal Medicine, the Ohio State University, Columbus, OH, USA.ORCID 0000-0003-1638-9228
Vidyani SuryadevaraDepartment of Radiology, Molecular Imaging Program at Stanford, Stanford University, Stanford, CA, USA.
Xiaonan FuDepartment of Biochemistry, Institute for Protein Design and Graduate Program in Biological Physics, Structure and Design, University of Washington, Seattle, WA, USA.
Ziv Bar-JosephComputational Biology Department, School of Computer Science, Carnegie Mellon University, Pittsburgh, PA, USA.
Zhicheng JiDivision of Pulmonary, Allergy, and Critical Care Medicine, Department of Medicine and Department of Biostatistics and Bioinformatics, Duke University School of Medicine, Durham, NC, USA.
João F PassosDepartment of Physiology and Biomedical Engineering, Robert and Arlene Kogod Center on Aging, Mayo Clinic, Rochester, MN, USA. passos.joao@mayo.edu.ORCID 0000-0001-8765-1890
University of Minnesota · USUniversity of Pittsburgh · USBuck Institute for Research on Aging · USBrown University · USMayo Clinic in Florida · USCarnegie Mellon University · USDuke University · USLung Institute · USNew York Genome Center · USSanford Burnham Prebys Medical Discovery Institute · USStanford University · USUniversity of Washington · USJackson Laboratory · USMayo Clinic in Arizona · USUniversity of California San Diego · USUniversity of Michigan · USYale University · US
Funding
SenNet Supplement - Consortium BenchmarkingU24CA268108 · NCI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Philip D. Blood, JONATHAN C. SILVERSTEIN · 2021 to 2026
$22.1MTriState SenNET (Lung and Heart) Tissue Map and Atlas consortiumU54AG075931 · NIA · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI TOREN FINKEL, Melanie Koenigshoff · 2021 to 2026
$14.0MThe KAPP-Sen Tissue Mapping Center CollaborativeU54AG075941 · NIA · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI KUCHEL, GEORGE A · 2021 to 2025
$13.8MData Analysis CoreU54AG076040 · NIA · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI PHATNANI, HEMALI · 2021 to 2025
$13.1MThe Duke Senescent Cell Evaluations in Normal Tissues (SCENT) Mapping CenterU54AG075936 · NIA · DUKE UNIVERSITY · PI NIXON, ANDREW B. · 2021 to 2025
$12.7MSenescent cell mapping, identification and validation for human somatic and reproductive tissuesU54AG075932 · NIA · BUCK INSTITUTE FOR RESEARCH ON AGING · PI Simon Melov, Birgit Schilling · 2021 to 2026
$12.5MSpatial Mapping Senescent Cells Across the Mouse Lifespan by Multiplex Transcriptomics and EpigenomicsU54AG079758 · NIA · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · PI PETER D. ADAMS · 2022 to 2026
$12.1MMinnesota Tissue Mapping Center for Senescent CellsU54AG076041 · NIA · UNIVERSITY OF MINNESOTA · PI LAURA Jane NIEDERNHOFER · 2021 to 2026
$10.5MSeq-Scope: Microscopic Examination of Spatial Single Cell Transcriptome in Cell and Tissue SenescenceUH3CA268091 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI LEE, JUN HEE · 2023 to 2025
$1.7MDevelopment of machine learning software to quantitatively map telomere induced senescence in tissue sections during agingUH3CA268103 · NCI · MAYO CLINIC ROCHESTER · PI PASSOS, JOAO · 2023 to 2025
$1.6MDevelopment of machine learning software to quantitatively map telomere induced senescence in tissue sections during agingUG3CA268103 · NCI · MAYO CLINIC ROCHESTER · PI PASSOS, JOAO · 2021 to 2022
$1.1MSeq-Scope: Microscopic Examination of Spatial Single Cell Transcriptome in Cell and Tissue SenescenceUG3CA268091 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI LEE, JUN HEE · 2021 to 2022
$1.1MNCI NIH HHS U24 CA268108NCI NIH HHS UG3 CA268091NCI NIH HHS UG3 CA268096NCI NIH HHS UG3 CA268103NCI NIH HHS UG3 CA268112NCI NIH HHS UG3 CA268202NCI NIH HHS UH3 CA268091NCI NIH HHS UH3 CA268103NIA NIH HHS U54 AG075931NIA NIH HHS U54 AG075932NIA NIH HHS U54 AG075936NIA NIH HHS U54 AG075941NIA NIH HHS U54 AG076040NIA NIH HHS U54 AG076041NIA NIH HHS U54 AG079758
Abstract
Cellular senescence is a well-established driver of aging and age-related diseases. There are many challenges to mapping senescent cells in tissues such as the absence of specific markers and their relatively low abundance and vast heterogeneity. Single-cell technologies have allowed unprecedented characterization of senescence; however, many methodologies fail to provide spatial insights. The spatial component is essential, as senescent cells communicate with neighboring cells, impacting their function and the composition of extracellular space. The Cellular Senescence Network (SenNet), a National Institutes of Health (NIH) Common Fund initiative, aims to map senescent cells across the lifespan of humans and mice. Here, we provide a comprehensive review of the existing and emerging methodologies for spatial imaging and their application toward mapping senescent cells. Moreover, we discuss the limitations and challenges inherent to each technology. We argue that the development of spatially resolved methods is essential toward the goal of attaining an atlas of senescent cells.
Indexed as
AgingCellular SenescenceAnimalsHumansLongevityMiceUnited States
Identifiers
What OpenQuestion holds
Textmetadata
LicenceTDM
Read underepoch 390
Registered trials
None linked
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.