ArticleNature chemistry2023
Site-specific bioorthogonal protein labelling by tetrazine ligation using endogenous β-amino acid dienophiles.
Article in Nature chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- Achieving cell-type-specific bioorthogonal chemistry using enzyme-activated caged tetrazines.Nature chemical biology · 2026Article
- Reactive oxygen species-activated bioorthogonal chemistry in living systems enabled by boronate-caged dihydrotetrazines.Nature communications · 2026Article
- A Versatile Enzymatic Pathway for Modification of Peptide C‑Termini.ACS central science · 2025Article
- Multi-postpolymerization Functionalization of PLA Enabled by Sulfide Tetrazines as a Single End-Group Moiety.ACS polymers Au · 2025Article
- Investigation of Proteome-Tetrazine Reactivity for a Highly Selective Tetrazine Ligation in Live Cells.ACS central science · 2025Article
- Peptide Backbone Editing via Post-Translational O to C Acyl Shift.Journal of the American Chemical Society · 2025Article
- Mechanistic Insights Into Post-Translational α-Keto-β-Amino Acid Formation by a Radical S-Adenosyl Methionine Peptide Splicease.Angewandte Chemie (International ed. in English) · 2025Article
- Metagenomic study of lake microbial mats reveals protease-inhibiting antiviral peptides from a core microbiome member.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Bioorthogonal Janus microparticles for photothermal and chemo-therapy.Smart medicine · 2024Article
- Structural Transformations of Metal-Organic Cages through Tetrazine-Alkene Reactivity.Journal of the American Chemical Society · 2024Article
- Repurposing a plant peptide cyclase for targeted lysine acylation.Nature chemistry · 2024Article
- Bio-inspired biorthogonal compartmental microparticles for tumor chemotherapy and photothermal therapy.Journal of nanobiotechnology · 2024Article
- ifDEEPre: large protein language-based deep learning enables interpretable and fast predictions of enzyme commission numbers.Briefings in bioinformatics · 2024Article
- Genome Mining for New Enzyme Chemistry.ACS catalysis · 2024Review
- Bioorthogonal Reactions in Bioimaging.Topics in current chemistry (Cham) · 2024Review
- Deep Learning-Driven Library Design for theACS central science · 2023Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The tetrazine ligation is an inverse electron-demand Diels-Alder reaction widely used for bioorthogonal modifications due to its versatility, site specificity and fast reaction kinetics. A major limitation has been the incorporation of dienophiles in biomolecules and organisms, which relies on externally added reagents. Available methods require the incorporation of tetrazine-reactive groups by enzyme-mediated ligations or unnatural amino acid incorporation. Here we report a tetrazine ligation strategy, termed TyrEx (tyramine excision) cycloaddition, permitting autonomous dienophile generation in bacteria. It utilizes a unique aminopyruvate unit introduced by post-translational protein splicing at a short tag. Tetrazine conjugation occurs rapidly with a rate constant of 0.625 (15) M
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.