ArticleJournal of advanced research2024
Exogenous abscisic acid represses rice flowering via SAPK8-ABF1-Ehd1/Ehd2 pathway.
Article in Journal of advanced research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- OsbZIP60 Positively Regulates Salt-Stress Tolerance in Rice.International journal of molecular sciences · 2026Article
- Natural variation inScience advances · 2026Article
- Molecular Crosstalk Between Flowering Time and Drought Adaptation in Cereal Crops.Plants (Basel, Switzerland) · 2026Review
- Regulatory dynamics of molecular determinants in divergent rice varieties under the influence of nano-structured Zinc: an integrative study of OsbZIP genes and their targeting miRNAs.BMC plant biology · 2026Article
- Multi-Omics Analysis Sheds Light on the Relative Roles of Hormones and Nutrients in Regulating Secondary Flowering inPlants (Basel, Switzerland) · 2026Article
- MiABI5-like7-Horticulture research · 2026Article
- Far-Red Light Regulates the Circadian Rhythm Pathway to Accelerate Rice Flowering.International journal of molecular sciences · 2026Article
- "Bud to bloom"-hormonal coordination in floral initiation.Plant biology (Stuttgart, Germany) · 2025Review
- Identification and characterization of the TmSnRK2 family proteins related to chicoric acid biosynthesis in Taraxacum mongolicum.BMC genomics · 2025Article
- The transcription factor CCT30 promotes rice preharvest sprouting by regulating sugar signalling to inhibit the ABA-mediated pathway.Plant biotechnology journal · 2025Article
- A Series of Novel Alleles ofPlants (Basel, Switzerland) · 2025Article
- Research Progress on Photoperiod Gene Regulation of Heading Date in Rice.Current issues in molecular biology · 2024Review
- Epigenetics and plant hormone dynamics: a functional and methodological perspective.Journal of experimental botany · 2024Review
- The characteristic analysis of TaTDF1 reveals its function related to male sterility in wheat (Triticum aestivum L.).BMC plant biology · 2024Article
- RNA NNature reviews. Urology · 2024Review
- Comprehensive Review on Bimolecular Fluorescence Complementation and Its Application in Deciphering Protein-Protein Interactions in Cell Signaling Pathways.Biomolecules · 2024Review
- Diversification of FT-like genes in the PEBP family contributes to the variation of flowering traits in Sapindaceae species.Molecular horticulture · 2024Article
- Comprehensive analysis of stress-activated protein kinase genes (OsSAPKs) in rice flowering time.Planta · 2024Article
- The Association betweenInternational journal of molecular sciences · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
18 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionRice flowering is a major agronomic trait, determining yield and ecological adaptability in particular regions. ABA plays an essential role in rice flowering, but the underlying molecular mechanism remains largely elusive.
objectivesIn this study, we demonstrated a "SAPK8-ABF1-Ehd1/Ehd2" pathway, through which exogenous ABA represses rice flowering in a photoperiod-independent manner.
methodsWe generated abf1 and sapk8 mutants using the CRISPR-Cas9 method. Using yeast two-hybrid, Pull down, BiFC and kinase assays, SAPK8 interacted and phosphorylated ABF1. ABF1 directly bound to the promoters of Ehd1 and Ehd2 using ChIP-qPCR, EMSA, and LUC transient transcriptional activity assay, and suppressed the transcription of these genes.
resultsUnder both long day and short day conditions, simultaneous knock-out of ABF1 and its homolog bZIP40 accelerated flowering, while SAPK8 and ABF1 over-expression lines exhibited delayed flowering and hypersensitivity to ABA-mediated flowering repression. After perceiving the ABA signal, SAPK8 physically binds to and phosphorylates ABF1 to enhance its binding to the promoters of master positive flowering regulators Ehd1 and Ehd2. Upon interacting with FIE2, ABF1 recruited PRC2 complex to deposit H3K27me3 suppressive histone modification on Ehd1 and Ehd2 to suppress these genes transcription, thereby leading to later flowering.
conclusionOur work highlighted the biological functions of SAPK8 and ABF1 in ABA signaling, flowering control and the involvement of a PRC2-mediated epigenetic repression mechanism in the transcription regulation governed by ABF1 on ABA-mediated rice flowering repression.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.