Evidence map›Paper›PMID 37398492›Full record

ArticlemedRxiv : the preprint server for health sciences2023

Metagenomic sequencing detects human respiratory and enteric viruses in air samples collected from congregate settings.

Nicholas R Minor, Mitchell D Ramuta, Miranda R Stauss, Olivia E Harwood, Savannah F Brakefield, Alexandra Alberts, William C Vuyk, Max J Bobholz, Jenna R Rosinski, Sydney Wolf and 6 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in medRxiv : the preprint server for health sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors at 3 institutions in 1 country.

Nicholas R MinorWisconsin National Primate Research Center, Madison, WI USA.ORCID 0000-0003-2929-8229
Mitchell D RamutaDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, USA.
Miranda R StaussWisconsin National Primate Research Center, Madison, WI USA.
Olivia E HarwoodDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, USA.
Savannah F BrakefieldDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, USA.
Alexandra AlbertsDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, USA.
William C VuykDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, USA.
Max J BobholzDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, USA.
Jenna R RosinskiDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, USA.
Sydney WolfDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, USA.
Madelyn LundDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, USA.
Madison MussaDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, USA.
Lucas J BeversdorfCity of Milwaukee Health Department Laboratory, Milwaukee, WI.
Matthew T AliotaDepartment of Veterinary and Biomedical Sciences, University of Minnesota, Twin Cities, Minneapolis, MN, USA.
Shelby L O'ConnorWisconsin National Primate Research Center, Madison, WI USA.
David H O'ConnorWisconsin National Primate Research Center, Madison, WI USA.
University of Wisconsin–Madison · USMilwaukee Health Department · USUniversity of Minnesota · US

Funding

Microbial Pathogenesis &Host Responses Training ProgramT32AI055397 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI KLEIN, BRUCE STEVEN · 2003 to 2024
$5.5M
Anticipating and rapidly responding to respiratory virus outbreaks with continuous air sampling in K-12 schoolsR01AI170737 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI David H. O'Connor, SHELBY L OCONNOR · 2023 to 2026
$3.1M
NIAID NIH HHS R01 AI170737NIAID NIH HHS T32 AI055397
6 · The paper itself

Abstract

Innovative methods for evaluating virus risk and spread, independent of test-seeking behavior, are needed to improve routine public health surveillance, outbreak response, and pandemic preparedness. Throughout the COVID-19 pandemic, environmental surveillance strategies, including wastewater and air sampling, have been used alongside widespread individual-based SARS-CoV-2 testing programs to provide population-level data. These environmental surveillance strategies have predominantly relied on pathogen-specific detection methods to monitor viruses through space and time. However, this provides a limited picture of the virome present in an environmental sample, leaving us blind to most circulating viruses. In this study, we explore whether pathogen-agnostic deep sequencing can expand the utility of air sampling to detect many human viruses. We show that sequence-independent single-primer amplification sequencing of nucleic acids from air samples can detect common and unexpected human respiratory and enteric viruses, including influenza virus type A and C, respiratory syncytial virus, human coronaviruses, rhinovirus, SARS-CoV-2, rotavirus, mamastrovirus, and astrovirus.

Identifiers

PMID37398492
PMCPMC10312882
OpenAlexW4379016651

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.