Evidence map›Paper›PMID 37398233›Full record

ArticlebioRxiv : the preprint server for biology2023

Resolving conformational changes that mediate a two-step catalytic mechanism in a model enzyme.

Jack B Greisman, Kevin M Dalton, Dennis E Brookner, Margaret A Klureza, Candice J Sheehan, In-Sik Kim, Robert W Henning, Silvia Russi, Doeke R Hekstra

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 6 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Jack B GreismanDepartment of Molecular & Cellular Biology, Harvard University, Cambridge, MA, United States.
Kevin M DaltonDepartment of Molecular & Cellular Biology, Harvard University, Cambridge, MA, United States.
Dennis E BrooknerDepartment of Molecular & Cellular Biology, Harvard University, Cambridge, MA, United States.
Margaret A KlurezaDepartment of Chemistry & Chemical Biology, Harvard University, Cambridge, MA, United States.
Candice J SheehanDepartment of Molecular & Cellular Biology, Harvard University, Cambridge, MA, United States.
In-Sik KimBioCARS, The University of Chicago, Argonne National Laboratory, Lemont, IL, United States.
Robert W HenningBioCARS, The University of Chicago, Argonne National Laboratory, Lemont, IL, United States.
Silvia RussiStanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA, United States.
Doeke R HekstraDepartment of Molecular & Cellular Biology, Harvard University, Cambridge, MA, United States.
Harvard University · USArgonne National Laboratory · USSLAC National Accelerator Laboratory · US

Funding

X-ray Absorption Spectroscopy (XAS) pp.711-759P41GM103393 · NIGMS · STANFORD UNIVERSITY · PI HODGSON, KEITH O · 2012 to 2019
$30.6M
Electric Field-stimulated Protein MechanicsP41GM118217 · NIGMS · UNIVERSITY OF CHICAGO · PI RANGANATHAN, RAMA · 2019 to 2021
$5.3M
Identifying Principles of Protein Mechanics by Applying Force and Observing MotionDP2GM141000 · NIGMS · HARVARD UNIVERSITY · PI HEKSTRA, DOEKE ROMKE · 2020 to 2020
$2.4M
NIGMS NIH HHS DP2 GM141000NIGMS NIH HHS P41 GM103393NIGMS NIH HHS P41 GM118217
6 · The paper itself

Abstract

Enzymes catalyze biochemical reactions through precise positioning of substrates, cofactors, and amino acids to modulate the transition-state free energy. However, the role of conformational dynamics remains poorly understood due to lack of experimental access. This shortcoming is evident with

Indexed as

AllosteryCatalysisConformational SelectionDHFRExcited StatesProtein DynamicsX-ray Crystallography

Identifiers

PMID37398233
PMCPMC10312612
OpenAlexW4379191710

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.